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Doubly Caged Linker for AND-Type Fluorogenic Construction of Protein/Antibody Bioconjugates and In Situ Quantification 期刊论文

编号：

5deb015b-ae49-407e-bd6d-be40b7081220
作者：

Liu, Guhuan^[1];Shi, Guohai(施国海)^[2]Sheng, Haoyue^[2];Jiang, Yanyan^[1];Liang, Haojun^[1];Liu, Shiyong^[1];
语种：

English
期刊：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION ISSN：1433-7851 2017 年 56 卷 30 期 (8686 - 8691) ; JUL 17
收录：
关键词：

bioconjugates click chemistry fluorescent probes FRET prodrugs
摘要：

In situ quantification of the conjugation efficiency of azide-terminated synthetic polymers/imaging probes and thiol-functionalized antibodies/proteins/peptides was enabled by a doubly caged profluorescent and heterodifunctional core molecule C1 as a self-sorting bridging unit. Orthogonal dual "click" coupling of C1 with azide-and thiol-functionalized precursors led to highly fluorescent bioconjugates, whereas single-click products remained essentially nonfluorescent. Integration with FRET processes was also possible. For the construction of antibody-probe conjugates from an anti-carcinoembryonic antigen and a quinone-caged profluorescent naphthalimide derivative, the dual "click" coupling process with C1 was monitored on the basis of the emission turn-on of C1, whereas prominent changes in FRET ratios occurred for antibody-imaging-probe conjugates when specifically triggered by quinone oxidoreductase (NQO1), which is overexpressed in various types of cancer cells.
推荐引用方式
GB/T 7714：

Liu Guhuan,Shi Guohai,Sheng Haoyue, et al. Doubly Caged Linker for AND-Type Fluorogenic Construction of Protein/Antibody Bioconjugates and In Situ Quantification [J].ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(30):8686-8691.
APA：

Liu Guhuan,Shi Guohai,Sheng Haoyue,Jiang Yanyan,&Liu Shiyong.(2017).Doubly Caged Linker for AND-Type Fluorogenic Construction of Protein/Antibody Bioconjugates and In Situ Quantification .ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(30):8686-8691.
MLA：

Liu Guhuan, et al. "Doubly Caged Linker for AND-Type Fluorogenic Construction of Protein/Antibody Bioconjugates and In Situ Quantification" .ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56,30(2017):8686-8691.

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