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1 alpha,25-Dihydroxyvitamin D3 Induces Neutrophil Apoptosis through the p38 MAPK Signaling Pathway in Chronic Obstructive Pulmonary Disease Patients 期刊论文

编号：

5fd06e4f-10f7-4fdc-86e3-a45c4ab95e59
作者：

Yang, Haihua(杨海华)#^[1]Long, Feng(龙丰)^[1]Zhang, Youzhi(张有志)^[1]Yu, Ronghuan^[2];Zhang, Peng^[1];Li, Wenjing^[1];Li, Shuijun^[3];Jin, Xianqiao(金先桥)^[4]Xia, Jingwen(夏敬文)^[4]Dong, Liang(董樑)^[4]Zhu, Ning(朱柠)^[4]Huang, Ying^[2];Gong, Yi(龚益)*^[4]Chen, Xiaodong(陈小东)^[4]
语种：

English
期刊：

PLoS One ISSN：1932-6203 2015 年 10 卷 4 期 ; APR 23
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摘要：

Background Reduced neutrophil apoptosis plays an important role in the pathogenesis of acute exacerbation chronic obstructive pulmonary disease (AECOPD). The p38 mitogen-activated protein kinase (MAPK) signaling pathway is involved in neutrophil apoptosis. 1 alpha,25-Dihydroxyvitamin D3 (1 alpha,25VitD3) can induce tumor cell apoptosis. The aim of this study was to assess the effects of 1 alpha,25VitD3 on peripheral blood neutrophil apoptosis in AECOPD and examine the role of the p38 MAPK signaling pathway. Methods The study enrolled 36 AECOPD patients and 36 healthy volunteers. Venous blood samples were obtained from both groups. Serum 25-hydroxyvitamin D (25-(OH) D) levels in peripheral venous blood were assayed using liquid chromatography-tandem mass spectrometry (LC-MS/MS); the neutrophils were separated and cultured with SB203580 (a p38 inhibitor) and 1 alpha,25VitD3. Neutrophil apoptosis was measured using flow cytometry, and phosphop-38 MAPK protein expression was detected by Western blot. Statistical analysis was performed using analysis of variance. Student's t-test and Pearson's correlation coefficient were used for the between-group differences and correlation analysis, respectively. Results The 25-(OH) D levels were lower in AECOPD patients than in healthy controls, and the peripheral blood neutrophil apoptosis results were similar. 1 alpha,25VitD3 increased the apoptosis rate and the level of phospho-p38 MAPK in peripheral blood neutrophils of AECOPD patients. SB203580 partly inhibited 1 alpha,25VitD3-induced peripheral blood neutrophil apoptosis and phospho-p38 MAPK overexpression. The 25-(OH) D levels were positively correlated with increased peripheral blood neutrophil apoptosis and phospho-p38 MAPK levels. In addition, expression of the phospho-p38 MAPK protein was also positively correlated with peripheral blood neutrophil apoptosis. Conclusion Our results suggest that 1 alpha,25VitD3 induces peripheral blood neutrophil apoptosis through the p38 MAPK signaling pathway in AECOPD patients.
推荐引用方式
GB/T 7714：

Yang Haihua,Long Feng,Zhang Youzhi, et al. 1 alpha,25-Dihydroxyvitamin D3 Induces Neutrophil Apoptosis through the p38 MAPK Signaling Pathway in Chronic Obstructive Pulmonary Disease Patients [J].PLoS One,2015,10(4).
APA：

Yang Haihua,Long Feng,Zhang Youzhi,Yu Ronghuan,&Chen Xiaodong.(2015).1 alpha,25-Dihydroxyvitamin D3 Induces Neutrophil Apoptosis through the p38 MAPK Signaling Pathway in Chronic Obstructive Pulmonary Disease Patients .PLoS One,10(4).
MLA：

Yang Haihua, et al. "1 alpha,25-Dihydroxyvitamin D3 Induces Neutrophil Apoptosis through the p38 MAPK Signaling Pathway in Chronic Obstructive Pulmonary Disease Patients" .PLoS One 10,4(2015).

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