Osteonecrosis of the femoral head (ONFH), namely avascular necrosis, is caused by osteocyte death and collapse of the articular surface resulting from decreased vascular supply to the subchondral bone of the femoral head. Current, the pathogenesis and etiology of osteonecrosis of the femoral head have not been revealed completely. In this study, we build an ONFH rat model by buthionine sulphoximine (BSO) treatment, the microRNA profiling of ONFH were obtained by next-generation sequencing technology. Totally 24 microRNAs were identified dysregulated in ONFH models. MicroRNA target gene prediction shows that these miRNAs are associated with 763 downstream genes. Pathway enrichment analysis reveals that these target genes are related to GnRH signaling pathway, and MAPK signaling pathway. The coexpression analysis of target genes identified several PSMB genes and MAPK genes. Moreover, we observed the five relatively abundant microRNAs show strong correlation with serum Glutathione concentration. In summary, our study described the microRNA profile characteristic of ONFH, which provides new sights into understanding of the pathogenesis of ONFH. Also our results suggest that changes in the miRNA expression in ONFH may provide potential biomarkers for ONFH prognosis.
[1]Fudan Univ, Dept Orthoped, Huashan Hosp, Shanghai 200433, Peoples R China.
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