首页 / 院系成果 / 成果详情页

Robo1 promotes angiogenesis in hepatocellular carcinoma through the Rho family of guanosine triphosphatases' signaling pathway 期刊论文

编号：

68929264-4529-48c4-ac93-afc4c40957f9
作者：

Ao, JianYang^[1,2,3,5];Chai, ZongTao(柴宗涛)^[1,2,3,6]Zhang, YuanYuan^[1,2,3];Zhu, XiaoDong(朱小东)^[1,2,3]Kong, LingQun^[4];Zhang, Ning^[1,2,3];Ye, BoGen(叶伯根)^[1,2,3]Cai, Hao(蔡浩)^[1,2,3]Gao, Dongmei(高冬梅)^[1,2,3]Sun, HuiChuan(孙惠川)^[1,2,3]
语种：

English
期刊：

TUMOR BIOLOGY ISSN：1010-4283 2015 年 36 卷 11 期 (8413 - 8424) ; NOV
收录：
关键词：

Hepatocellular carcinoma Angiogenesis Roundabout receptors 1 Rho GTPase
摘要：

Robo1 is a member of the Robo immunoglobulin superfamily of proteins, and it plays an important role in angiogenesis and cancer. In this study, we investigate the role of roundabout 1 (Robo1) in tumor angiogenesis in hepatocellular carcinoma (HCC). Firstly, the relationship between Robo1 expression on tumors and patient's survival and endothelial cells in tumor blood vessels and patient's survival was studied. Secondly, Robo1 was overexpressed or knocked down in human umbilical vein endothelial cells (HUVECs). Cell proliferation, motility, and tube formation were compared in HUVEC with different Robo1 expression. Also, HUVECs with different Robo1 expression were mixed with HCCLM3 and HepG2 hepatoma cells and then implanted in a nude mouse model to examine the effects of Robo1 in endothelial cells on tumor growth and angiogenesis. Cell motility-related molecules were studied to investigate the potential mechanism how Robo1 promoted tumor angiogenesis in HCC. The disease-free survival of the patients with high Robo1 expression in tumoral endothelial cells was significantly shorter than that of those with low expression (P = 0.021). Overexpression of Robo1 in HUVECs resulted in increased proliferation, motility, and tube formation in vitro. In the implanted mixture of tumor cells and HUVECs with an increased Robo1 expression, tumor growth and microvessel density were enhanced compared with controls. Robo1 promoted cell division cycle 42 (Cdc42) expression in HUVECs, and a distorted actin cytoskeleton in HUVECs was observed when Robo1 expression was suppressed. In conclusion, Robo1 promoted angiogenesis in HCC mediated by Cdc42.
推荐引用方式
GB/T 7714：

Ao Jian-Yang,Chai Zong-Tao,Zhang Yuan-Yuan, et al. Robo1 promotes angiogenesis in hepatocellular carcinoma through the Rho family of guanosine triphosphatases' signaling pathway [J].TUMOR BIOLOGY,2015,36(11):8413-8424.
APA：

Ao Jian-Yang,Chai Zong-Tao,Zhang Yuan-Yuan,Zhu Xiao-Dong,&Sun Hui-Chuan.(2015).Robo1 promotes angiogenesis in hepatocellular carcinoma through the Rho family of guanosine triphosphatases' signaling pathway .TUMOR BIOLOGY,36(11):8413-8424.
MLA：

Ao Jian-Yang, et al. "Robo1 promotes angiogenesis in hepatocellular carcinoma through the Rho family of guanosine triphosphatases' signaling pathway" .TUMOR BIOLOGY 36,11(2015):8413-8424.

浏览次数：4  下载次数：0

分享到：

浏览次数：4

下载次数：0

打印次数：0