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Enhanced transfection of polyplexes based on pluronic-polypropylenimine dendrimer for gene transfer 期刊论文

编号：

6b82ee0a-e99d-4d0d-b5cd-d41bdf2f17e4
作者：

Hao, Junguo(郝俊国)#^[1]Sha, Xianyi(沙先谊)*^[1]Tang, Yuanjia^[2,3];Jiang, Ye^[1];Zhang, Zhiwen(张志文)^[1]Zhang, Wei(张伟)^[1]Li, Yajuan(李雅娟)^[1]Fang, Xiaoling(方晓玲)^[1]
语种：

English
期刊：

ARCHIVES OF PHARMACAL RESEARCH ISSN：0253-6269 2009 年 32 卷 7 期 (1045 - 1054) ; JUL
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关键词：

Pluronic Polypropylenimine Polyplex Nanoparticles Non-viral vector Gene delivery system
摘要：

Third generation cationic dendritic polymeric polypropyleneimine (PPI) was modified by Pluronic P123 and investigated for gene delivery. The cytotoxicity of P123-PPI was evaluated by the MTT assay and shown to be much lower than that of PPI alone. P123-PPI and PPI can both condense plasmid DNA into nanoparticles with a size of approximately 100 nm and a zeta potential of about 15 mV at the N/P ratio 20:1. The nanoparticles can protect plasmid DNA from being digested by DNase I at a concentration of 0.4 U/mu g DNA. The nanoparticles were resistant to dissociation induced by 50% fetal bovine serum and 75 mu g/mL sodium heparin. The transfection efficiency of SPC-A1 cells using P123-PPI/DNA nanoparticles was much higher than the transfection utilizing PPI/DNA nanoparticles. The addition of free P123 during the preparation of P123-PPI/DNA nanoparticles could significantly enhance the transfection efficiency in the presence of 10% fetal bovine serum. Therefore, P123-PPI/DNA complex nanoparticles may be a safe, efficient and promising cationic conjugate for gene delivery.
推荐引用方式
GB/T 7714：

Hao Junguo,Sha Xianyi,Tang Yuanjia, et al. Enhanced transfection of polyplexes based on pluronic-polypropylenimine dendrimer for gene transfer [J].ARCHIVES OF PHARMACAL RESEARCH,2009,32(7):1045-1054.
APA：

Hao Junguo,Sha Xianyi,Tang Yuanjia,Jiang Ye,&Fang Xiaoling.(2009).Enhanced transfection of polyplexes based on pluronic-polypropylenimine dendrimer for gene transfer .ARCHIVES OF PHARMACAL RESEARCH,32(7):1045-1054.
MLA：

Hao Junguo, et al. "Enhanced transfection of polyplexes based on pluronic-polypropylenimine dendrimer for gene transfer" .ARCHIVES OF PHARMACAL RESEARCH 32,7(2009):1045-1054.

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