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miR-204-5p Inhibits Proliferation and Invasion and Enhances Chemotherapeutic Sensitivity of Colorectal Cancer Cells by Downregulating RAB22A 期刊论文

编号：

6e8440ea-8563-4dda-8381-c66d4dbef00d
作者：

Yin, Yuan^[1];Zhang, Binbin^[1,2];Wang, Weili^[3];Fei, Bojian^[3];Quan, Chao^[1];Zhang, Jiwei^[1];Song, Mingxu^[1];Bian, Zehua^[1];Wang, Qifeng(王奇峰)^[4]Ni, Shujuan(倪淑娟)^[4]Hu, Yaling^[1];Mao, Yong^[1];Zhou, Leyuan^[1];Wang, Yugang^[5];Yu, Jian^[6];Du, Xiang(杜祥)^[4]Hua, Dong^[1];Huang, Zhaohui^[1];
语种：

English
期刊：

CLINICAL CANCER RESEARCH ISSN：1078-0432 2014 年 20 卷 23 期 (6187 - 6199) ; DEC 1
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摘要：

Purpose: miR-204-5p was found to be downregulated in colorectal cancer tissues in our preliminary microarray analyses. However, the function of miR-204-5p in colorectal cancer remains unknown. We therefore investigated the role, mechanism, and clinical significance of miR-204-5p in colorectal cancer development and progression. Experimental Design: We measured the expression of miR-204-5p and determined its correlation with patient prognoses. Ectopic expression in colorectal cancer cells, xenografts, and pulmonary metastasis models was used to evaluate the effects of miR-204-5p on proliferation, migration, and chemotherapy sensitivity. Luciferase assay and Western blotting were performed to validate the potential targets of miR-204-5p after the preliminary screening by a microarray analysis and computer-aided algorithms. Results: miR-204-5p is frequently downregulated in colorectal cancer tissues, and survival analysis showed that the downregulation of miR-204-5p in colorectal cancer was associated with poor prognoses. Ectopic miR-204-5p expression repressed colorectal cancer cell growth both in vitro and in vivo. Moreover, restoring miR-204-5p expression inhibited colorectal cancer migration and invasion and promoted tumor sensitivity to chemotherapy. Mechanistic investigations revealed that RAB22A, a member of the RAS oncogene family, is a direct functional target of miR-204-5p in colorectal cancer. Furthermore, RAB22A protein levels in colorectal cancer tissues were frequently increased and negatively associated with miR-204-5p levels and survival time. Conclusions: Our results demonstrate for the first time that miR-204-5p acts as a tumor suppressor in colorectal cancer through inhibiting RAB22A and reveal RAB22A to be a new oncogene and prognostic factor for colorectal cancer. (C) 2014 AACR.
推荐引用方式
GB/T 7714：

Yin Yuan,Zhang Binbin,Wang Weili, et al. miR-204-5p Inhibits Proliferation and Invasion and Enhances Chemotherapeutic Sensitivity of Colorectal Cancer Cells by Downregulating RAB22A [J].CLINICAL CANCER RESEARCH,2014,20(23):6187-6199.
APA：

Yin Yuan,Zhang Binbin,Wang Weili,Fei Bojian,&Huang Zhaohui.(2014).miR-204-5p Inhibits Proliferation and Invasion and Enhances Chemotherapeutic Sensitivity of Colorectal Cancer Cells by Downregulating RAB22A .CLINICAL CANCER RESEARCH,20(23):6187-6199.
MLA：

Yin Yuan, et al. "miR-204-5p Inhibits Proliferation and Invasion and Enhances Chemotherapeutic Sensitivity of Colorectal Cancer Cells by Downregulating RAB22A" .CLINICAL CANCER RESEARCH 20,23(2014):6187-6199.

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