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Lysine 88 Acetylation Negatively Regulates Ornithine Carbamoyltransferase Activity in Response to Nutrient Signals 期刊论文

编号：

6f2c2f43-2745-42b2-8505-b953371dfa05
作者：

Yu, Wei^[1,2];Lin, Yan(林彦)^[2]Yao, Jun(姚鋆)^[2]Huang, Wei^[1,2];Lei, Qunying(雷群英)^[2,3]Xiong, Yue^[2,4];Zhao, Shimin(赵世民)^[1,2]Guan, KunLiang^[2,5,6];
语种：

English
期刊：

JOURNAL OF BIOLOGICAL CHEMISTRY ISSN：0021-9258 2009 年 284 卷 20 期 (13669 - 13675) ; MAY 15
收录：
摘要：

Ornithine carbamoyltransferase (OTC) is a key enzyme in the urea cycle to detoxify ammonium produced from amino acid catabolism. OTC deficiency is an X-linked genetic disorder ranging from fatal in newborns to hyperammonemia and anorexia in adults. Through affinity purification of acetylated peptides and mass spectrometry, we identified that OTC is acetylated on lysine residues, including Lys(88), which is also mutated in OTC-deficient patients. OTC acetylation was confirmed to occur under physiological conditions. Biochemical characterizations revealed that OTC Lys(88) acetylation decreases the affinity for carbamoyl phosphate, one of the two OTC substrates, and the maximum velocity, whereas the Km for ornithine, the other OTC substrate, is not affected. Furthermore, Lys(88) acetylation is regulated by both extracellular glucose and amino acid availability, indicating that OTC activity may be regulated by cellular metabolic status. Our results provide an example of the novel mechanism of regulating metabolic enzyme activity through protein acetylation.
推荐引用方式
GB/T 7714：

Yu Wei,Lin Yan,Yao Jun, et al. Lysine 88 Acetylation Negatively Regulates Ornithine Carbamoyltransferase Activity in Response to Nutrient Signals [J].JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(20):13669-13675.
APA：

Yu Wei,Lin Yan,Yao Jun,Huang Wei,&Guan Kun-Liang.(2009).Lysine 88 Acetylation Negatively Regulates Ornithine Carbamoyltransferase Activity in Response to Nutrient Signals .JOURNAL OF BIOLOGICAL CHEMISTRY,284(20):13669-13675.
MLA：

Yu Wei, et al. "Lysine 88 Acetylation Negatively Regulates Ornithine Carbamoyltransferase Activity in Response to Nutrient Signals" .JOURNAL OF BIOLOGICAL CHEMISTRY 284,20(2009):13669-13675.

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