首页 / 院系成果 / 成果详情页

Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter 期刊论文

编号：

6f5f7002-4fd4-4f8b-ae2a-57ce12d1f1ff
作者：

Ji, Haiyan^[1,2] Jiang, Zhengtao^[1,2] Lu, Panpan^[1,2] Ma, Li^[3] Liz, Chuan^[3] Pan, Hanyu^[1,2] Fill, Zheng^[1,2] Qui, Xiying^[1,2] Wang, Pengfei^[1,2] Deng, Junxiao^[1,2] Yang, Xinyi^[1,2] Wang, Jianhua^[3] Zhu, Huanzhang^[1,2]
语种：

English
期刊：

MOLECULAR THERAPY ISSN：1525-0016 2016 年 24 卷 3 期 (508 - 521) ; MAR
收录：
摘要：

HIV-1 escapes antiretroviral agents by integrating into the host DNA and forming a latent transcriptionally silent HIV-1 provirus. Transcriptional activation is prerequisite for reactivation and the eradication of latent HIV-1 pro viruses. dCas9-SunTag-VP64 transcriptional system has been reported that it can robustly activate the expression of an endogenous gene,using a single guide RNA (sgRNA). Here, we systematically investigated the potential of dCas9-SunTag-VP64 with the designed sgRNAs for reactivating latent HIV-1. We found dCas9-SunTag-VP64 with sgRNA 4 or sgRNA 5 targeted from 164 to 146 or -124 to -106 bp upstream of the transcription start sites of HIV-1 could induce high expression of luciferase reporter gene after screening of sgRNAs targeting different regions of the HIV-1 promoter. Further, we confirmed that dCas9-SunTag-VP64 with sgRNA 4 or sgRNA 5 can effectively reactivate latent HIV-1 transcription in several latently infected human T-cell lines. Moreover, we confirmed that the reactivation of latent HIV-1 by dCas9-SunTag-VP64 with the designed sgRNA occurred through specific binding to the HIV-1 LTR promoter without genotoxicity and global T-cell activation. Taken together, our data demonstrated dCas9-SunTag-VP64 system can effectively and specifically reactivate latent HIV-1 transcription, suggesting that this strategy could offer a novel approach to anti-HIV-1 latency.
推荐引用方式
GB/T 7714：

Ji Haiyan,Jiang Zhengtao,Lu Panpan, et al. Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter [J].MOLECULAR THERAPY,2016,24(3):508-521.
APA：

Ji Haiyan,Jiang Zhengtao,Lu Panpan,Ma Li,&Zhu Huanzhang.(2016).Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter .MOLECULAR THERAPY,24(3):508-521.
MLA：

Ji Haiyan, et al. "Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter" .MOLECULAR THERAPY 24,3(2016):508-521.

浏览次数：9  下载次数：0

分享到：

浏览次数：9

下载次数：0

打印次数：0