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Proteomic response of methicillin-resistant S. aureus to a synergistic antibacterial drug combination: a novel erythromycin derivative and oxacillin 期刊论文

编号：

6fe16e28-6a34-4901-82c1-1c2cf5472935
作者：

Liu, Xiaofen^[1,2,3] Pai, PeiJin^[1,4,5] Zhang, Weipeng^[6] Hu, Yingwei^[1] Dong, Xiaojing^[7] Qian, Peiyuan^[6] Chen, Daijie^[7,8] Lam, Henry^[1,9]
语种：

English
期刊：

SCIENTIFIC REPORTS ISSN：2045-2322 2016 年 6 卷 ; JAN 25
收录：
摘要：

The use of antibacterial drug combinations with synergistic effects is increasingly seen as a critical strategy to combat multi-drug resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA). In this work, the proteome responses in MRSA under the stress of a sub-inhibitory dose of a synergistic drug combination of a novel erythromycin derivative, SIPI-8294, and oxacillin, were studied by label-free quantitative proteomics. Several control treatment groups were designed to isolate proteome responses potentially related to the synergy: (1) the non-synergistic drug combination of erythromycin and oxacillin, (2) SIPI-8294 only, (3) oxacillin only and (4) erythromycin only. Results showed that 200 proteins were differentially expressed in SIPI-8294/oxacillin-treated cells. Among these proteins, the level of penicillin binding protein 2a, the protein mainly responsible for oxacillin resistance in MRSA, was four times lower in the SIPI-8294/oxacillin group than in the erythromycin/oxacillin group, suggesting that SIPI-8294 may interfere with this known oxacillin resistance mechanism. Moreover, hierarchical clustering analysis of differentially expressed proteins under different treatments revealed that SIPI-8294/oxacillin elicits very different responses than the individual drugs or the non-synergistic erythromycin/oxacillin combination. Bioinformatic analysis indicated that the synergistic effect can be further traced to a disruption in oxidation-reduction homeostasis and cell wall biosynthesis.
推荐引用方式
GB/T 7714：

Liu Xiaofen,Pai Pei-Jin,Zhang Weipeng, et al. Proteomic response of methicillin-resistant S. aureus to a synergistic antibacterial drug combination: a novel erythromycin derivative and oxacillin [J].SCIENTIFIC REPORTS,2016,6.
APA：

Liu Xiaofen,Pai Pei-Jin,Zhang Weipeng,Hu Yingwei,&Lam Henry.(2016).Proteomic response of methicillin-resistant S. aureus to a synergistic antibacterial drug combination: a novel erythromycin derivative and oxacillin .SCIENTIFIC REPORTS,6.
MLA：

Liu Xiaofen, et al. "Proteomic response of methicillin-resistant S. aureus to a synergistic antibacterial drug combination: a novel erythromycin derivative and oxacillin" .SCIENTIFIC REPORTS 6(2016).

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