[1]Soochow University, Department of Molecular Diagnostics and Biopharmaceutics,Suzhou,China
[2]Wuhan University, Department of Engineering Mechanics,Wuhan,China
[3]Southeast University, State Key Laboratory of Bioelectronics,Nanjing,China
[4]Fudan University Shanghai Cancer Center, Department of Radiation Oncology,Shanghai,China
[5]China Academy of Engineering Physics, Laboratory for Shock Wave and Detonation Physics Research,Chengdu,China
[6]Soochow University, College of Pharmaceutical Sciences,Suzhou,China
CCR5 is a coreceptor for the entry of HIV into CD4+ cells and some of its natural mutants possess potential protection against HIV-1 infection. Individuals with the CCR5-Δ32 genotype have a lower possibility of being infected with HIV and take longer to progress to AIDS if infected. The present study demonstrated a new primer extension assay for genetic analysis of the CCR5del32 mutation: each genotype is determined by a specific number of fragments amplified. Quick and efficient screening and testing of this specific genotype is clinically useful in epidemiological study, risk analysis, and determining donors for allogenic stem cell transplantation. Copyright ? 2011 American Scientific Publishers All rights reserved.