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Multiplex profiling of glycoproteins using a novel bead-based lectin array 期刊论文

编号：

73f7a477-efe8-489a-89f6-60704e00d172
作者：

Wang, Hong^[1,2,3];Li, Hong^[1,2];Zhang, Wei^[1,2];Wei, Liming^[2];Yu, Hongxiu(余红秀)*^[2]Yang, Pengyuan(杨芃原)^[1,2]
语种：

English
期刊：

PROTEOMICS ISSN：1615-9853 2014 年 14 卷 1 期 (78 - 86) ; JAN
收录：
关键词：

Bead-based lectin array Glycan profiling Glycoproteomics Multiplexed assay Sensitivity
摘要：

Lectin array is becoming important in profiling targeted glycan/glycoprotein, but weak interaction between lectin and glycan causes low sensitivity of the approach. This study aims to develop a bead-based lectin array for improving the sensitivity of glycosylation profiling. Lectins are chemically coupled to fluorescent dye coated microbeads, and glycan-lectin recognition is carried out three dimensionally. The performance of this platform was evaluated, and the LOD of lectin Ricinus communis agglutinin 120 (RCA120) was 50 pg/mL (1 pM) of asialofetuin, providing the bead-based lectin microarray with the highest sensitivity among the reported lectin microarrays. Furthermore, multiplexed assay was performed, which allowed the simultaneous detection of multiple carbohydrate epitopes in a single reaction vessel. The glycosylation patterns of hepatocellular carcinoma associated immunoglobulin G were analyzed, and increased (-1,6) core fucosylation and (-2,6) sialylation patterns were observed, which may provide significant clinical evidence for disease diagnosis.
推荐引用方式
GB/T 7714：

Wang Hong,Li Hong,Zhang Wei, et al. Multiplex profiling of glycoproteins using a novel bead-based lectin array [J].PROTEOMICS,2014,14(1):78-86.
APA：

Wang Hong,Li Hong,Zhang Wei,Wei Liming,&Yang Pengyuan.(2014).Multiplex profiling of glycoproteins using a novel bead-based lectin array .PROTEOMICS,14(1):78-86.
MLA：

Wang Hong, et al. "Multiplex profiling of glycoproteins using a novel bead-based lectin array" .PROTEOMICS 14,1(2014):78-86.

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