Background: This study aimed to investigate the effects of treatment with recombinant interleukin-15 (IL-15) on T cells, natural killer (NK) cells, and interferon-gamma (IFN-gamma) on the immune response in a rat cecal ligation and perforation model of sepsis.
Material/Methods: Sprague-Dawley rats (n=120) were divided into four groups (n=30). A rat model of clinical sepsis was created using cecal ligation and perforation, and 109 rats successfully developed sepsis. Rats were then injected intraperitoneally with 0.5, 1.0, and 1.5 mu g of recombinant rat IL-15 or saline. Survival was determined, and the numbers of T cells and NK cells, and the expression levels of IL-15 and IFN-gamma were detected in the peripheral blood of rats in each group at 24 h and 48 h.
Results: The levels of IL-15 and IFN-gamma, as well as the numbers of T cells and NK cells, were significantly increased in the IL-15-treated groups compared with the control group at both 24 h and 48 h (P<0.05). Levels of IL-15 and IFN-gamma were significantly increased in the IL-15-treated groups at 48 h compared with 24 h in the control group. Levels of IL-15, the numbers of T cells and NK cells, and the levels of IFN-gamma in peripheral blood were significantly lower at 48 h when compared with 24 h (P<0.05).
Conclusions: In a rat model of sepsis, treatment with recombinant IL-15 significantly increased T cell and NK cell numbers, and levels of IFN-gamma, and prolonged the survival of rats with sepsis.
[1]Shanghai Jiao Tong Univ, Dept Crit Care Med, Renji Hosp, Sch Med, Shanghai, Peoples R China;
[2]Shanghai Jiao Tong Univ, Dept Tradit Chinese Med, Renji Hosp, Sch Med, Shanghai, Peoples R China;
[3]Fudan Univ, Dept Hepat Surg, Shanghai Canc Ctr, Shanghai, Peoples R China;
[4]Anhui Univ, Dept Gastroenterol, Shidong Hosp, Sch Med, Hefei, Anhui, Peoples R China
(8.0+极速模式)