While it is known that miR-203 is frequently downregulated in many types of human cancer, little is known regarding its expression and functional role in colorectal cancer (CRC). In this study, we aimed to investigate the expression and the potential mechanisms of miR-203 in colorectal cancer. MiR-203 was significantly downregulated in CRC tissues compared with matched normal adjacent tissues. Our clinical data show that decreased miR-203 was associated with an advanced clinical tumor-node-metastasis stage, lymph node metastasis, and poor survival in CRC patients. Furthermore, externally induced expression of miR-203 significantly inhibited CRC cell proliferation and invasion in vitro and in vivo. Mechanistically, we identified EIF5A2 as a direct and functional target of miR-203. The levels of miR-203 were inversely correlated with levels of the EIF5A2 in the CRC tissues. Restoration of EIF5A2 in the miR-203-overexpressing CRC cells reversed the suppressive effects of miR-203. Our results demonstrate that miR-203 serves as a tumor suppressor gene and may be useful as a new potential therapeutic target in CRC.
[1]Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Gen Surg, 100 Haining Rd, Shanghai 200080, Peoples R China.
[2]TongJi Univ, Tianyou Hosp, Dept Gastroenterol, 500 Zhennan Rd, Shanghai 200331, Peoples R China.
[3]TongJi Univ, Shanghai Peoples Hosp 10, Dept Nucl Med, 301 Yanchang Rd, Shanghai 200072, Peoples R China.
[4]Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Gastroenterol, 100 Haining Rd, Shanghai 200080, Peoples R China.
[5]Fudan Univ, Zhongshan Hosp, Dept Gen Surg, 180 Xietu Rd, Shanghai 200032, Peoples R China.
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