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Rapid and sensitive profiling and quantification of the human cell line proteome by LC-MS/MS without prefractionation 期刊论文

编号：

79630ca7-ed25-41c3-b77a-a6c1e360c9fc
作者：

Yin, Xuefei(殷薛飞)#Liu, Xiaohui;Zhang, Yang;Yan, Guoquan;Wang, Fang;Lu, Haojie;Shen, Huali;Yang, Pengyuan(杨芃原)^[1,2]
语种：

English
期刊：

PROTEOMICS ISSN：1615-9853 2014 年 14 卷 17-18 期 (2008 - 2016) ; SEP
收录：
关键词：

1D LC-MS/MS High throughput Rapid quantification Technology
摘要：

In this paper, we demonstrate a rapid and reproducible 1D LC-MS/MS workflow for fast quantitative proteomic research. We have optimized the LC-MS/MS conditions, including digestion and gradient conditions, sample loading amount, and MS parameter settings. As a result, we were able to obtain twice as many protein identifications compared with the LC-MS/MS conditions before optimization. More than 4500 protein groups and 50 000 peptides were identified in less than 8 h without any fractionation. This 1D workflow was then applied to the analysis of the MLN4924 treated/untreated human umbilical vein endothelial cell (HUVEC) samples with label-free quantification. In these experiments, a total of 179 proteins showed a statistically significant expression change after the MLN4924 treatment. Functional analysis showed that these proteins are associated with cell death and survival; gene expression; cell cycle; and DNA replication, recombination, and repair.
推荐引用方式
GB/T 7714：

Yin Xuefei,Liu Xiaohui,Zhang Yang, et al. Rapid and sensitive profiling and quantification of the human cell line proteome by LC-MS/MS without prefractionation [J].PROTEOMICS,2014,14(17-18):2008-2016.
APA：

Yin Xuefei,Liu Xiaohui,Zhang Yang,Yan Guoquan,&Yang Pengyuan.(2014).Rapid and sensitive profiling and quantification of the human cell line proteome by LC-MS/MS without prefractionation .PROTEOMICS,14(17-18):2008-2016.
MLA：

Yin Xuefei, et al. "Rapid and sensitive profiling and quantification of the human cell line proteome by LC-MS/MS without prefractionation" .PROTEOMICS 14,17-18(2014):2008-2016.

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