Chronic ambient fine particulate matter (PM2.5) exposure correlates with various adverse health outcomes. Its impact on the circulating metabolome-a comprehensive functional readout of the interaction between an organism's genome and environment-has not however been fully understood. This study thus performed metabolomics analyses using a chronic PM2.5 exposure mouse model. C57BI/6J mice (female) were subjected to inhalational concentrated ambient PM2.5 (CAP) or filtered air (FA) exposure for 10 months. Their sera were then analyzed by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). These analyses identified 2570 metabolites in total, and 148 of them were significantly different between FA- and CAP-exposed mice. The orthogonal partial least-squares discriminant analysis (OPLS-DA) and heatmap analyses displayed evident clustering of FA and CAP-exposed samples. Pathway analyses identified 6 perturbed metabolic pathways related to amino acid metabolism. In contrast, biological characterization revealed that 71 differential metabolites were related to lipid metabolism. Furthermore, our results showed that CAP exposure increased stress hormone metabolites, 18-oxocortisol and 5a-tetrahydrocortisol, and altered the levels of circadian rhythm biomarkers including melatonin, retinal and 5-methoxytryptophol. (C) 2019 Elsevier Ltd. All rights reserved.
[1]Fudan Univ, Sch Publ Hlth, Dept Environm Hlth, Shanghai 200032, Peoples R China;
[2]Shanghai Meteorol Serv, Shanghai Key Lab Meteorol & Hlth, Shanghai, Peoples R China;
[3]Univ Maryland, Sch Med, Dept Med, Div Cardiol, Baltimore, MD 21201 USA;
[4]Nanjing Med Univ, Affiliated Huaian Peoples Hosp 1, Dept Pathol, Huaian, Peoples R China;
[5]Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Dept Intervent & Vasc Surg, Shanghai, Peoples R China;
[6]Fudan Univ, Reprod & Dev Res Inst, Shanghai 200032, Peoples R China;
[7]Fudan Univ, Zhongshan Hosp, Dept Neurol, Shanghai 200032, Peoples R China
(8.0+极速模式)