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Direct interaction between surface beta 1,4-galactosyltransferase 1 and epidermal growth factor receptor (EGFR) inhibits EGFR activation in hepatocellular carcinoma 期刊论文

编号：

7a2c270a-e6cd-4b4e-a6ac-8cc0bf43a12a
作者：

Tang, Wenqing^[1];Weng, Shuqiang(翁书强)^[1]Zhang, Si^[2];Wu, Weibing^[2];Dong, Ling(董玲)^[1]Shen, Xizhong(沈锡中)^[1]Zhang, Songwen^[2];Gu, Jianxin^[2];Xue, Ruyi(薛如意)*^[1]
语种：

English
期刊：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ISSN：0006-291X 2013 年 434 卷 3 期 (449 - 454) ; MAY 10
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关键词：

beta 1,4GT1 EGFR Interaction Dimerization Phosphorylation
摘要：

Our previous studies showed that cell surface beta 1,4-galactosyltransferase 1 (beta 1,4GT1) negatively regulated cell survival through inhibition and modulation of the epidermal growth factor receptor (EGFR) signaling pathway in human hepatocellular carcinoma (HCC) SMMC-7721 cells. However, the underlying mechanism remains unclear. Here we demonstrated that beta 1,4-galactosyltransferase 1 (beta 1,4GT1) interacted with EGFR in vitro by GST pull-down analysis. Furthermore, we demonstrated that beta 1,4GT1 bound to EGFR in vivo by co-immunoprecipitation and determined the co-localization of beta 1,4GT1 and EGFR on the cell surface via confocal laser scanning microscopy analysis. Finally, using I-125-EGF binding experiments and Western blot analysis, we found that overexpression of beta 1,4GT1 inhibited I-125-EGF binding to EGFR, and consequently reduced the levels of EGFR dimerization and phosphorylation. In contrast, RNAi-mediated knockdown of beta 1,4GT1 increased the levels of EGFR dimerization and phosphorylation. These data suggest that cell surface beta 1,4GT1 interacts with EGFR and inhibits EGFR activation. (C) 2013 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Tang Wenqing,Weng Shuqiang,Zhang Si, et al. Direct interaction between surface beta 1,4-galactosyltransferase 1 and epidermal growth factor receptor (EGFR) inhibits EGFR activation in hepatocellular carcinoma [J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,434(3):449-454.
APA：

Tang Wenqing,Weng Shuqiang,Zhang Si,Wu Weibing,&Xue Ruyi.(2013).Direct interaction between surface beta 1,4-galactosyltransferase 1 and epidermal growth factor receptor (EGFR) inhibits EGFR activation in hepatocellular carcinoma .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,434(3):449-454.
MLA：

Tang Wenqing, et al. "Direct interaction between surface beta 1,4-galactosyltransferase 1 and epidermal growth factor receptor (EGFR) inhibits EGFR activation in hepatocellular carcinoma" .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 434,3(2013):449-454.

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