Eosinophil infiltration, a hallmark of allergic asthma, is essential for type 2 immune responses. How the initial eosinophil recruitment is regulated by lung dendritic cell (DC) subsets during the memory stage after allergen challenge is unclear. Here, we show that the initial eosinophil infiltration is dependent on lung cDC1s, which require nitric oxide (NO) produced by inducible NO synthase from lung CD24(-)CD11b(+) DC2s for inducing CCL17 and CCL22 to attract eosinophils. During late phase responses after allergen challenge, lung CD24(+) cDC2s inhibit eosinophil recruitment through secretion of TGF-beta 1, which impairs the expression of CCL17 and CCL22. Our data suggest that different lung antigen-presenting cells modulate lung cDC1-mediated eosinophil recruitment dynamically, through secreting distinct soluble factors during the memory stage of chronic asthma after allergen challenge in the mouse.
[1]Taishan Med Univ, Inst Immunol, Tai An 271000, Shandong, Peoples R China;
[2]Taishan Med Univ, Sch Basic Med Sci, Tai An 271000, Shandong, Peoples R China;
[3]Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China;
[4]Shanghai Inst Biol Sci, Inst Hlth Sci, Shanghai 200031, Peoples R China
(8.0+极速模式)