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Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells 期刊论文

编号：

83b79dda-e04f-4bbb-b13e-8cf42fc5b0e3
作者：

Qiao, Jie^[1,2];Cui, ShuJian^[4];Xu, LeiLei^[1,2];Chen, SiJie^[5];Yao, Jun(姚鋆)^[1,2]Jiang, YingHua(姜英华)^[1,2]Peng, Gang(彭刚)^[5]Fang, CaiYun^[3];Yang, PengYuan(杨芃原)^[1,2,3]Liu, Feng(刘锋)^[1,2]
语种：

English
期刊：

ONCOTARGET ISSN：1949-2553 2015 年 6 卷 2 期 (1171 - 1189) ; JAN 20
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关键词：

Gastric cancer Proteomics Filamin C Metastasis Tumor suppressor
摘要：

Gastric cancer (GC) is the fourth and fifth most common cancer in men and women, respectively. We identified 2,750 proteins at false discovery rates of 1.3% (protein) and 0.03% (spectrum) by comparing the proteomic profiles of three GC and a normal gastric cell lines. Nine proteins were significantly dysregulated in all three GC cell lines, including filamin C, a muscle-specific filamin and a large actin-cross-linking protein. Downregulation of filamin C in GC cell lines and tissues were verified using quantitative PCR and immunohistochemistry. Data-mining using public microarray datasets shown that filamin C was significantly reduced in many human primary and metastasis cancers. Transient expression or silencing of filamin C affected the proliferation and colony formation of cancer cells. Silencing of endogenous filamin C enhanced cancer cell migration and invasion, whereas ectopic expression of filamin C had opposing effects. Silencing of filamin C increased the expression of matrix metallopeptidase 2 and improved the metastasis of prostate cancer in a zebrafish model. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. These findings establish a functional role of filamin C in human cancers and these data will be valuable for further study of its mechanisms.
推荐引用方式
GB/T 7714：

Qiao Jie,Cui Shu-Jian,Xu Lei-Lei, et al. Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells [J].ONCOTARGET,2015,6(2):1171-1189.
APA：

Qiao Jie,Cui Shu-Jian,Xu Lei-Lei,Chen Si-Jie,&Liu Feng.(2015).Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells .ONCOTARGET,6(2):1171-1189.
MLA：

Qiao Jie, et al. "Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells" .ONCOTARGET 6,2(2015):1171-1189.

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