Aim. Pigment epithelium-derived factor (PEDF) is pluripotent in both antiangiogenesis and direct tumor inhibition. We thus investigated role of PEDF in endometrial cancer cells in vitro. Methods. Exogenous PEDF was delivered into Ishikawa and KLE cells by adenovirus system. Realtime RT-PCR was used to detect expressional change of several angiogenesis-related factors. Proliferation and viability tests were performed to establish suitable transduction titre. Cell cycle and apoptosis assays were conducted to evaluate inhibitive effects of PEDF on tumor cell growth. Transwell tests were carried out to investigate the retardation of tumor cell invasiveness by PEDF. Results. PEDF induced decrease in viability, proliferation and invasiveness of both endometrial tumor cell lines. Incremented cell apoptosis and aggregated population in G1 phase of cell cycle was noted in PEDF groups. PEDF also induced down-regulation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) and up-regulation of thrombospondin-1 (TSP-1) in both cell lines. Conclusion. Adenovirus mediated PEDF is potent in retarding the growth and invasiveness of endometrial cancer cells. PEDF can become promising as novel therapeutic strategy in endometrial carcinoma.
[1]Fudan Univ, Dept Gynecol, Obstet & Gynecol Hosp, Shanghai 200011, Peoples R China.
(8.0+极速模式)