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Genome-Wide Analysis of Long Noncoding RNA (lncRNA) Expression in Hepatoblastoma Tissues 期刊论文

编号：

86196417-d778-4fe3-b057-7c08a0e7b64a
作者：

Dong, Rui^[1,2] Jia, Deshui^[3] Xue, Ping^[1,2] Cui, Ximao^[1,2] Li, Kai^[1,2] Zheng, Shan^[1,2] He, Xianghuo^[3] Dong, Kuiran^[1,2]
语种：

English
期刊：

PLOS ONE ISSN：1932-6203 2014 年 9 卷 1 期 ; JAN 17
收录：
摘要：

Long noncoding RNAs (lncRNAs) have crucial roles in cancer biology. We performed a genome-wide analysis of lncRNA expression in hepatoblastoma tissues to identify novel targets for further study of hepatoblastoma. Hepatoblastoma and normal liver tissue samples were obtained from hepatoblastoma patients. The genome-wide analysis of lncRNA expression in these tissues was performed using a 46180 K lncRNA microarray and Sureprint G3 Human lncRNA Chips. Quantitative RTPCR (qRT-PCR) was performed to confirm these results. The differential expressions of lncRNAs and mRNAs were identified through fold-change filtering. Gene Ontology (GO) and pathway analyses were performed using the standard enrichment computation method. Associations between lncRNAs and adjacent protein-coding genes were determined through complex transcriptional loci analysis. We found that 2736 lncRNAs were differentially expressed in hepatoblastoma tissues. Among these, 1757 lncRNAs were upregulated more than two-fold relative to normal tissues and 979 lncRNAs were downregulated. Moreover, in hepatoblastoma there were 420 matched lncRNA-mRNA pairs for 120 differentially expressed lncRNAs, and 167 differentially expressed mRNAs. The co-expression network analysis predicted 252 network nodes and 420 connections between 120 lncRNAs and 132 coding genes. Within this co-expression network, 369 pairs were positive, and 51 pairs were negative. Lastly, qRT-PCR data verified six upregulated and downregulated lncRNAs in hepatoblastoma, plus endothelial cell-specific molecule 1 (ESM1) mRNA. Our results demonstrated that expression of these aberrant lncRNAs could respond to hepatoblastoma development. Further study of these lncRNAs could provide useful insight into hepatoblastoma biology.
推荐引用方式
GB/T 7714：

Dong Rui,Jia Deshui,Xue Ping, et al. Genome-Wide Analysis of Long Noncoding RNA (lncRNA) Expression in Hepatoblastoma Tissues [J].PLOS ONE,2014,9(1).
APA：

Dong Rui,Jia Deshui,Xue Ping,Cui Ximao,&Dong Kuiran.(2014).Genome-Wide Analysis of Long Noncoding RNA (lncRNA) Expression in Hepatoblastoma Tissues .PLOS ONE,9(1).
MLA：

Dong Rui, et al. "Genome-Wide Analysis of Long Noncoding RNA (lncRNA) Expression in Hepatoblastoma Tissues" .PLOS ONE 9,1(2014).

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