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Rapid synthesis of titanium(IV)-immobilized magnetic mesoporous silica nanoparticles for endogenous phosphopeptides enrichment 期刊论文

编号：

8921b459-5ca1-4e91-9cb1-9a81dd46cab6
作者：

Yao, Jizong;Sun, Nianrong;Wang, Jiawen;Xie, Yiqin;Deng, Chunhui(邓春晖)*^[1,2]Zhang, Xiangmin(张祥民)
语种：

English
期刊：

PROTEOMICS ISSN：1615-9853 2017 年 17 卷 8 期 ; APR
收录：
关键词：

Endogenous phosphopeptides MALDI-TOF MS Mesoporous silica Size exclusive
摘要：

The MALDI-TOF MS has already been a main platform for phosphoproteome analysis. However, there are some weaknesses in direct analysis of endogenous phosphopeptides by MALDI-TOF MS because of the serious suppression effect and poor ionization efficiency, which is brought by the excess of nonphosphopeptides and protein. It is essential to enrich endogenous phosphopeptides from complex biosamples efficiently prior to MALDI-TOF MS analysis. Herein, we present a time-saving and detailed protocol for the synthesis of titanium(iv)immobilized magnetic mesoporous silica nanoparticles (denoted as Fe3O4@ mSiO(2)-Ti4+), the subsequent enrichment process, and MALDI-TOF MS analysis. We tested the LOD, size-exclusive effect, reproducibility, and stability of Fe3O4@ mSiO(2)-Ti4+ nanoparticles. Furthermore, the ability of this protocol for identifying endogenous phosphopeptides in healthy human serum and saliva was investigated.
推荐引用方式
GB/T 7714：

Yao Jizong,Sun Nianrong,Wang Jiawen, et al. Rapid synthesis of titanium(IV)-immobilized magnetic mesoporous silica nanoparticles for endogenous phosphopeptides enrichment [J].PROTEOMICS,2017,17(8).
APA：

Yao Jizong,Sun Nianrong,Wang Jiawen,Xie Yiqin,&Zhang Xiangmin.(2017).Rapid synthesis of titanium(IV)-immobilized magnetic mesoporous silica nanoparticles for endogenous phosphopeptides enrichment .PROTEOMICS,17(8).
MLA：

Yao Jizong, et al. "Rapid synthesis of titanium(IV)-immobilized magnetic mesoporous silica nanoparticles for endogenous phosphopeptides enrichment" .PROTEOMICS 17,8(2017).

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