Objective: To evaluate the effect of Lycium barbarum polysaccharide (LBP) on gastric cancer (GC) cells and to explore the associated mechanism. Methods: Human GC SGC-7901 cells were divided into control, 10 mu M LBP, 20 mu M LBP and 50 mu M LBP groups. CCK8 assay and Transwell assay were performed to evaluate the proliferation, migration and invasion of SGC-7901 cells. Western blotting was used to determine protein expressions. Results: The proliferation, migration and invasion decreased significantly in 20 mu M LBP and 50 mu M LBP groups. As the result of Western blotting, protein levels of MMP2, MMP9, Snail and vimentin decreased in 20 mu M LBP and 50 mu M LBP groups with different degrees. The expression E-cadherin significantly increased in all three experimental groups. The phosphorylation levels of AKT and PI3K in 20 mu M LBP and 50 mu M LBP groups were much lower than control group. Conclusion: LBP could inhibit the proliferation, migration and invasion of human GC cells bydown-regulation of MMPs and suppression of epithelial-mesenchymal transition (EMT).
[1]Fudan Univ, Minhang Hosp, Dept Gen Surg, Shanghai, Peoples R China.
[2]Shanghai Jiao Tong Univ, Dept Gen Surg, Peoples Hosp 6, 600 Yishan Rd, Shanghai 200030, Peoples R China.
(8.0+极速模式)