Objective: Condyloma acuminatum (CA) is a common, viral, sexually transmitted disease worldwide. Human papillomavirus (HPV) genotyping has important clinical implications for the treatment of CA. We developed a loop-mediated isothermal amplification (LAMP) method for the detection of HPV. Methods: We collected 294 cervical scrape samples, including 30 HPV-6-positive, 30 HPV-11-positive, 22 HPV-16-positive, 20 HPV-42-positve, 30 HPV-43-positive, 20 HPV-44-positive and 142 HPV-negative samples. Tissues from 40 patients with a pathological diagnosis of CA were paraffin-embedded and analyzed by LAMP and Luminex. Hydroxynaphthol blue (HNB) and electrophoresis were used to detect the results of LAMP. Results: LAMP and Luminex systems were compared in detecting six subtypes of HPV. LAMP reactions were specific for each subtype.The sensitivity of LAMP for HPV-6, as determined by the HNB indicator assay, was 1000 copies/tube. The kappa value between the two methods was 0.98 (HPV-6), 0.94 (HPV-11), 0.89 (HPV-43), 0.87 (HPV-42) 0.79 (HPV-16) and 0.68 (HPV-44). Among the 142 HPV-negative samples determined by the Luminex assay, HPV-6 was detected in eight and HPV-11 in one by LAMP. Among the 40 CA samples, the results of LAMP and Luminex were in agreement in 38 (95%). Conclusion:The results of this study indicated that the LAMP assay with HNB is superior to the Luminex method in terms of sensitivity and specificity. The specificity of LAMP was 100% and the sensitivity of LAMP was 1000 copies/tube using HNB. LAMP is therefore a useful, quick and accurate method for the clinical diagnosis of HPV subtypes.
[1]Tongji Univ, Shanghai Peoples Hosp 10, Dept Cent Lab, Shanghai, Peoples R China;
[2]Tongji Univ, Shanghai Peoples Hosp 10, Dept Lab Med, Shanghai, Peoples R China;
[3]Tongji Univ, Shanghai Skin Dis Hosp, Dept Lab Med, Shanghai, Peoples R China;
[4]Fudan Univ, Huashan Hosp, Dept Lab Med, Shanghai, Peoples R China;
[5]Tongji Univ, Shanghai Peoples Hosp 10, Dept Radiat Oncol, Shanghai, Peoples R China
(8.0+极速模式)