A ultra performance liquid chromatographic(UPLC)method with photo-diode array(PDA)detection for the quantification of vancomycin in human plasma was developed and validated.The method included an extraction of vancomycin by deproteinization with acetonitrile.The analyses were carried out at 230 nm as the detcet wavelength on a ACQUITY UPLC BEH C18 column(2.1 × 50 mm,1.7um)using a mobile phase composed of acetonitrile and 5 mM KH2PO4 buffer at pH 2.5.The calibration curve was linear over the range from 1 to 100 mg/L(R2 higher than 0.999).The method was validated via extraction recovery,intra-and inter-day methodological recovery and stability of vancomycin in serum.We used both established UPLC method and FPIA with vancomycin Kit to measure the serum vancomycin concentrations in 172 clinical samples,which were collected from the patients receiving vancomycin in the hospitals localized in Shanghai,China.Correlation analysis between the measurements of UPLC and FPIA in 172 serum samples gave the linear regression equation:(concentration by UPLC)=0.949*(concentration by FPIA)-0.517(R2 =0.923).Thanks to the short analysis time and small quantities of plasma needed,this UPLC method developed in this study can be easily used for vancomycin TDM.
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