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FoxO3a pathway 期刊论文

编号：

90a46272-364f-49a9-84b0-6c5248af6cda
作者：

Wang, Shuo(王硕)#^[1,2]Zhang, Chao(张潮)^[1,2]Niyazi, Sidikejiang^[1,2];Zheng, Long(郑龙)^[1,2]Li, Jiawei^[1,2];Zhang, Weitao(张伟韬)^[1,2]Xu, Ming(许明)^[1,2]Rong, Ruiming(戎瑞明)^[1,2,4]Yang, Cheng(杨橙)*^[1,2]Zhu, Tongyu(朱同玉)*^[1,2,3]
语种：

English
期刊：

JOURNAL OF TRANSLATIONAL MEDICINE ISSN：1479-5876 2017 年 15 卷 ; FEB 15
收录：
关键词：

Cyclic helix B peptide Mesenchymal stem cell Starvation Nrf2 Sirt3 FoxO3a
摘要：

Background: Mesenchymal stem cell (MSC) has been widely explored in the past decade as a cell-based treatment for various diseases. However, poor survival of adaptively transferred MSCs limits their clinical therapeutic potentials, which is largely ascribed to the nutrient starvation. In this study, we determined whether a novel kidney protective peptide CHBP could protect MSCs against starvation and invested the underlying mechanisms. Methods: MSCs were subjected to serum deprivation and CHBP of graded concentrations was administered. Cell viability and apoptosis were detected by CCK-8, Annexin V/PI assay and Hoechst staining. ROS generation, mitochondrial membrane potential indicated by JC-1 and mitochondrial mass were measured by flow cytometry. The location of cytochrome c within cells was observed under fluorescence microscopy. Expressions of Nrf2, Sirt3, and FoxO3a were analyzed by western blot. In addition, preconditioning MSCs with CHBP was applied to test the possible protection against starvation. Finally, the effect of CHBP on the differentiation and self-renewal capacity of MSCs was also examined. Results: CHBP improved cell viability and suppressed apoptosis in a dose dependent manner. Starvation resulted in the mitochondrial dysfunction and treatment of CHBP could alleviate mitochondrial stress by diminishing oxidative injury of ROS, restoring mitochondrial membrane potential and maintaining mitochondrial membrane integrity. Importantly, Nrf2/Sirt3/FoxO3a pathway was activated by CHBP and Sirt3 knockdown partially abolished the protection of CHBP. Moreover, MSCs pretreated with CHBP were more resistant to starvation. Under normal condition, CHBP exerted little effects on the differential and self-renewal capacity of MSCs. Conclusions: The present study demonstrated the efficient protection of CHBP upon MSCs against starvation-induced mitochondrial dysfunction and apoptosis and indicated possible involvement of Nrf2/Sirt3/FoxO3a pathway in the protective effect.
推荐引用方式
GB/T 7714：

Wang Shuo,Zhang Chao,Niyazi Sidikejiang, et al. A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway [J].JOURNAL OF TRANSLATIONAL MEDICINE,2017,15.
APA：

Wang Shuo,Zhang Chao,Niyazi Sidikejiang,Zheng Long,&Zhu Tongyu.(2017).A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway .JOURNAL OF TRANSLATIONAL MEDICINE,15.
MLA：

Wang Shuo, et al. "A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway" .JOURNAL OF TRANSLATIONAL MEDICINE 15(2017).

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