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Generation of conditional Acvrl1 knockout mice by CRISPR/Cas9-mediated gene targeting 期刊论文

编号：

962041e7-aa9c-46a0-a373-c48dd04a07f2
作者：

Xu, Ming^[1];Xu, Hongzhi(徐宏治)*^[2]Chen, Jian(陈坚)^[3]Chen, Chunjui^[2];Xu, Feng(徐锋)^[2]Qin, Zhiyong(秦智勇)^[2]
语种：

English
期刊：

MOLECULAR AND CELLULAR PROBES ISSN：0890-8508 2018 年 37 卷 (32 - 38) ; FEB
收录：
关键词：

CRISPR/Cas9 Acvrl1 Mouse Arteriovenous malformation
摘要：

Objectives: This study aimed to generate mutant mice containing the Acvrl1 gene flanked with LoxP sequences to allow conditional deletion of Acvrll by the LoxP/Cre system. Such mice may facilitate the development of brain arteriovenous malformation (BAVM) models. Methods: The CRISPR/Cas9 technique was used to edit Acvrl1. Two single guide RNAs (sgRNAs) with recognition sites on intron 3 and 8 and a donor vector that was homologous with the targeted gene and contained two LoxP sequences were designed and constructed. The in vitro-synthesized sgRNA, Cas9 mRNA and donor vectors were injected into mouse zygotes, which were then transferred into pseudopregnant mice. Neonatal mutant mice were identified by genotyping and sequencing. Results: Two mice with a floxed Acvrl1 allele were generated at a success rate of 8.7%. The target mice, which were healthy and fertile, were obtained through interbreeding. Conclusion: CRISPR/Cas9 is a reliable gene-editing tool, and is able to efficiently modify Acvrll and create the target mice.
推荐引用方式
GB/T 7714：

Xu Ming,Xu Hongzhi,Chen Jian, et al. Generation of conditional Acvrl1 knockout mice by CRISPR/Cas9-mediated gene targeting [J].MOLECULAR AND CELLULAR PROBES,2018,37:32-38.
APA：

Xu Ming,Xu Hongzhi,Chen Jian,Chen Chunjui,&Qin Zhiyong.(2018).Generation of conditional Acvrl1 knockout mice by CRISPR/Cas9-mediated gene targeting .MOLECULAR AND CELLULAR PROBES,37:32-38.
MLA：

Xu Ming, et al. "Generation of conditional Acvrl1 knockout mice by CRISPR/Cas9-mediated gene targeting" .MOLECULAR AND CELLULAR PROBES 37(2018):32-38.

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