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Efficacy and Persistence of Allogeneic Adipose-Derived Mesenchymal Stem Cells Combined with Hyaluronic Acid in Osteoarthritis After Intra-articular Injection in a Sheep Model 期刊论文

编号：

975a2945-e5e1-4289-be71-fd07b8973e91
作者：

Feng, Chenchen^[1];Luo, Xuan^[1];He, Na^[1];Xia, Huitang^[2,3];Lv, Xiaoteng^[1];Zhang, Xue^[2,3];Li, Dan^[2,3];Wang, Fei^[1];He, Jiaping^[1];Zhang, Li^[1];Lin, Xiangming^[4];Lin, Liping^[4];Yin, Huabin(尹化斌)^[4]He, Jiyin^[5];Wang, Jingwu^[6];Cao, Wei^[1];Wang, Richard^[1];Zhou, Guangdong^[2,3,7];Wang, Wen^[1];
语种：

English
期刊：

TISSUE ENGINEERING PART A ISSN：1937-3341 2018 年 24 卷 3-4 期 (219 - 233) ; FEB
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关键词：

adipose-derived mesenchymal stem cells allogeneic hyaluronic acid intra-articular injection osteoarthritis cell tracking superparamagnetic iron oxide sheep model
摘要：

Although a number of studies have reported efficacy of autologous adipose-derived mesenchymal stem cells (AD-MSCs) in treating osteoarthritis (OA) no reliable evidences demonstrate whether allogeneic AD-MSCs can efficiently block OA progression in a large animal model. This study explored the efficacy and survival of allogeneic AD-MSCs combined with hyaluronic acid (HA) after intra-articular (IA) injection in a sheep OA model, which were conventionally established by anterior cruciate ligament resection and medial meniscectomy. Allogeneic AD-MSCs from donor sheep at high (5x10(7) cells) and low (1x10(7) cells) doses combined with HA, HA alone, or saline alone were injected into the OA sheep at 3 and 6 weeks after surgery, respectively. Evaluations by magnetic resonance imaging (MRI), macroscopy, micro-computed tomography, and cartilage-specific staining demonstrated that AD-MSCs+HA treated groups preserved typical articular cartilage feature. Inflammatory factors from synovial fluid of AD-MSCs+HA treated groups were significantly lower than those in the HA alone group. Notably, transforming growth factor beta 1 and insulin-like growth factor 1 were detected in the supernatant of cultured AD-MSCs. In addition, labeling signals of allogeneic AD-MSCs could be detected by MRI after 14 weeks of injection and be found in synovium by histology. These results indicated that IA injection of allogeneic AD-MSCs combined with HA could efficiently block OA progression and promote cartilage regeneration and allogeneic AD-MSCs might survive at least 14 weeks after IA injection.
推荐引用方式
GB/T 7714：

Feng Chenchen,Luo Xuan,He Na, et al. Efficacy and Persistence of Allogeneic Adipose-Derived Mesenchymal Stem Cells Combined with Hyaluronic Acid in Osteoarthritis After Intra-articular Injection in a Sheep Model [J].TISSUE ENGINEERING PART A,2018,24(3-4):219-233.
APA：

Feng Chenchen,Luo Xuan,He Na,Xia Huitang,&Wang Wen.(2018).Efficacy and Persistence of Allogeneic Adipose-Derived Mesenchymal Stem Cells Combined with Hyaluronic Acid in Osteoarthritis After Intra-articular Injection in a Sheep Model .TISSUE ENGINEERING PART A,24(3-4):219-233.
MLA：

Feng Chenchen, et al. "Efficacy and Persistence of Allogeneic Adipose-Derived Mesenchymal Stem Cells Combined with Hyaluronic Acid in Osteoarthritis After Intra-articular Injection in a Sheep Model" .TISSUE ENGINEERING PART A 24,3-4(2018):219-233.

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