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Effect of adenovirus-mediated hepaCAM gene expression on cell cycle of bladder cancer cell lines 期刊论文

编号：

9906e40f-76ff-4e44-b7ba-0195a5a7ffed
作者：

Lü, Changkun[0]^[1] Liu, Qi[1]^[2] Ma, Feifei[2]^[3] Luo, Chunli[3]^[4] Xu, Xin[4]^[5]
地址：

[1]Chongqing Medical University, Key Laboratory of Chongqing Municipality,Chongqing,China
[2]
[3]Fudan University Shanghai Medical College, Department of Laboratory Medicine,Shanghai,China
[4]Chongqing Medical University,Chongqing,China
[5]Chongqing Medical University, Department of Neurology,Chongqing,China
语种：

中文
期刊：

Academic Journal of Second Military Medical University ISSN：0258-879X 2013 年 34 卷 10 期 (1151 - 1154)
收录：
关键词：

Adenovirus Cell cycle CyclinD1 hepaCAM Urinary bladder neoplasms
摘要：

Objective: our study was to investigate the effect of hepaCAM gene on cell cycle of bladder cancer cell line T24 and BIU-87. Method: Adenovirus vector with hepaCAM was infected into T24 and BIU-87 cells. The expression of hepaCAM mRNA and protein were measured byRT-QPCR and Western-blot. Immunofluorescence detected the cellular localization of hepaCAM. The distribution of cell cycle was analyzed by FCM. The protein expression of cyclinD1 was measured by Westein-blot. Result: The expression of hepaCAM mRNA and protein was increased in two cell lines infected with pAdH5-hepaCAM. The G0/G1 cycle was increased in infected cells, and the protein expression of cyclinD1 was decreased in same cells. Immunofluorescence revealed that hepaCAM protein localized on cytoplasm in well-spread cells, otherwise it localized on the junction of each cell. Conclusion: T24 and BIU-87 cells that infected pAdH5- hepaCAM was blocked in G0/G1 cycle, and it can decreased the protein expression of cyclinD1.
推荐引用方式
GB/T 7714：

Lü Changkun/55748071300[0],Liu Qi/56805335700[1],Ma Feifei/56806101300[2], 等. Effect of adenovirus-mediated hepaCAM gene expression on cell cycle of bladder cancer cell lines [J].Academic Journal of Second Military Medical University,2013,34(10):1151-1154.
APA：

Lü Changkun/55748071300[0],Liu Qi/56805335700[1],Ma Feifei/56806101300[2],Luo Chunli/13907826200[3],&Xu Xin/55619297219[4].(2013).Effect of adenovirus-mediated hepaCAM gene expression on cell cycle of bladder cancer cell lines .Academic Journal of Second Military Medical University,34(10):1151-1154.
MLA：

Lü Changkun/55748071300[0], et al. "Effect of adenovirus-mediated hepaCAM gene expression on cell cycle of bladder cancer cell lines" .Academic Journal of Second Military Medical University 34,10(2013):1151-1154.

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