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In-depth proteomic profiling of left ventricular tissues in human end-stage dilated cardiomyopathy 期刊论文

编号：

9e59467e-7add-4e64-96cc-3f0e09968cd8
作者：

Liu, Shanshan^[1,2,3,4];Xia, Yan^[6];Liu, Xiaohui^[5];Wang, Yi(汪怡)^[1,2,3,4]Chen, Zhangwei(陈章炜)^[6]Xie, Juanjuan^[1,2,3,4];Qian, Juying(钱菊英)*^[6]Shen, Huali(申华莉)*^[1,2,3,4,5]Yang, Pengyuan(杨芃原)*^[1,2,3,4,5]
语种：

English
期刊：

ONCOTARGET ISSN：1949-2553 2017 年 8 卷 29 期 (48321 - 48332) ; JUL 18
收录：
关键词：

dilated cardiomyopathy left ventricle cell death S100A1 eEF2
摘要：

Dilated cardiomyopathy (DCM) is caused by reduced left ventricular (LV) myocardial function, which is one of the most common causes of heart failure (HF). We performed iTRAQ-coupled 2D-LC-MS/MS to profile the cardiac proteome of LV tissues from healthy controls and patients with end-stage DCM. We identified 4263 proteins, of which 125 were differentially expressed in DCM tissues compared to LV controls. The majority of these were membrane proteins related to cellular junctions and neuronal metabolism. In addition, these proteins were involved in membrane organization, mitochondrial organization, translation, protein transport, and cell death process. Four key proteins involved in the cell death process were also detected by western blotting, indicated that cell death was activated in DCM tissues. Furthermore, S100A1 and eEF2 were enriched in the "cellular assembly and organization" and "cell cycle" networks, respectively. We verified decreases in these two proteins in end-stage DCM LV samples through multiple reaction monitoring (MRM). These observations demonstrate that our understanding of the mechanisms underlying DCM can be deepened through comparison of the proteomes of normal LV tissues with that from end-stage DCM in humans.
推荐引用方式
GB/T 7714：

Liu Shanshan,Xia Yan,Liu Xiaohui, et al. In-depth proteomic profiling of left ventricular tissues in human end-stage dilated cardiomyopathy [J].ONCOTARGET,2017,8(29):48321-48332.
APA：

Liu Shanshan,Xia Yan,Liu Xiaohui,Wang Yi,&Yang Pengyuan.(2017).In-depth proteomic profiling of left ventricular tissues in human end-stage dilated cardiomyopathy .ONCOTARGET,8(29):48321-48332.
MLA：

Liu Shanshan, et al. "In-depth proteomic profiling of left ventricular tissues in human end-stage dilated cardiomyopathy" .ONCOTARGET 8,29(2017):48321-48332.

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