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MicroRNA expression profiles of granulocytic myeloid-derived suppressor cells from mice bearing Lewis lung carcinoma 期刊论文

编号：

9f79860a-9cab-4fd9-b9d0-c6c7a01766b5
作者：

Jiang, Jingwei(蒋京伟)#^[1,2]Gao, Qingmin^[1,2];Wang, Tian^[1,2];Lin, Hao(林浩)^[1,2]Zhan, Qiong(詹琼)^[1,2]Chu, Zhaohui(初钊辉)^[1,2]Huang, Ruofan(黄若凡)^[1,2]Zhou, Xinli(周鑫莉)^[1,2]Liang, Xiaohua(梁晓华)^[1,2]Guo, Weijian(郭伟剑)^[2,3]
语种：

English
期刊：

MOLECULAR MEDICINE REPORTS ISSN：1791-2997 2016 年 14 卷 5 期 (4567 - 4574) ; NOV
收录：
关键词：

myeloid-derived suppressor cells microRNA immunoregulation tumor immune bioinformatics analysis
摘要：

Myeloid-derived suppressor cells (MDSCs) are a group of heterogeneous myeloid cells that can suppress antitumor immunity. MDSCs are divided into granulocytic (G-MDSCs) and monocytic subsets. In the present study, the microRNA profiles of the G-MDSCs were determined and the differential expression of microRNAs between G-MDSCs from tumor-bearing mice and tumor-free mice was examined. The number of G-MDSCs in spleens of Lewis lung carcinoma (LLC)-bearing mice was similar to 6-fold higher than in spleens of normal mice (13.54 +/- 1.74% vs. 2.14 +/- 1.44%; P<0.01) and G-MDSCs account for about 72.9% of all MDSCs. The microRNA (miRNA) profiles of the G-MDSCs from spleen of LLC-bearing mice were obtained using a microRNA microarray and compared with their counterparts from spleens of tumor-free mice. A total of 43 miRNAs with >1.3-fold increased or decreased change were differentially expressed between the experimental and control group mice. The levels of nine of these differentially expressed miRNAs, miRNA-468 (miR-486), miR-192, miR-128, miR-125a, miR-149, miR-27a, miR-125b, miR-350 and miR-328, were also analyzed by RT-qPCR to validate the microarray data. The concordance rate between the results tested by the two methods was 88.9%. Bioinformatics analyses revealed that these miRNAs may act on various target genes, including Adar, Pik3r1, Rybp and Rabgap1, to regulate the survival, differentiation and the function of tumor-induced granulocytic MDSCs. The results revealed microRNAs and potential targets that may be vital for regulating survival, differentiation and function of G-MDSCs induced by LLC. Further investigation should be performed to clarify the roles of these microRNAs in regulating LLC-induced granulocytic MDSCs and the target genes that mediate their functions.
推荐引用方式
GB/T 7714：

Jiang Jingwei,Gao Qingmin,Wang Tian, et al. MicroRNA expression profiles of granulocytic myeloid-derived suppressor cells from mice bearing Lewis lung carcinoma [J].MOLECULAR MEDICINE REPORTS,2016,14(5):4567-4574.
APA：

Jiang Jingwei,Gao Qingmin,Wang Tian,Lin Hao,&Guo Weijian.(2016).MicroRNA expression profiles of granulocytic myeloid-derived suppressor cells from mice bearing Lewis lung carcinoma .MOLECULAR MEDICINE REPORTS,14(5):4567-4574.
MLA：

Jiang Jingwei, et al. "MicroRNA expression profiles of granulocytic myeloid-derived suppressor cells from mice bearing Lewis lung carcinoma" .MOLECULAR MEDICINE REPORTS 14,5(2016):4567-4574.

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