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Coupling microchip electrophoresis with MALDI-TOF-MS based on a freezing technique 期刊论文

编号：

a315fdf7-371d-4fc2-8f68-df3b47190a36
作者：

Nie, Lei^[1];Xu, GuoBin(徐国宾)^[1,2]Wang, XiaoYan^[1];Liu, Yun^[1];Yang, PengYuan(杨芃原)^[1,2]
语种：

English
期刊：

CHINESE CHEMICAL LETTERS ISSN：1001-8417 2013 年 24 卷 6 期 (491 - 493) ; JUN
收录：
关键词：

Microchip electrophoresis MALDI-TOF-MS Peptide analysis Freezing technique
摘要：

A freezing technique protocol was proposed for coupling microchip electrophoresis with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The microfluidic flow was frozen immediately after electrophoresis on microfluidic chip and the separated analyte molecules were kept in their zone pattern in the electrophoresis. Then, the frozen-chip was lyophilized and sent into TOF-MS instrument as a MALDI target, and the analyte molecules in the microfluidic channels were subjected to analysis by mass spectrometry. This approach could eliminate sample cross-contamination, providing a new interface for microchip electrophoresis and MALDI-MS. (C) 2013 Peng-Yuan Yang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
推荐引用方式
GB/T 7714：

Nie Lei,Xu Guo-Bin,Wang Xiao-Yan, et al. Coupling microchip electrophoresis with MALDI-TOF-MS based on a freezing technique [J].CHINESE CHEMICAL LETTERS,2013,24(6):491-493.
APA：

Nie Lei,Xu Guo-Bin,Wang Xiao-Yan,Liu Yun,&Yang Peng-Yuan.(2013).Coupling microchip electrophoresis with MALDI-TOF-MS based on a freezing technique .CHINESE CHEMICAL LETTERS,24(6):491-493.
MLA：

Nie Lei, et al. "Coupling microchip electrophoresis with MALDI-TOF-MS based on a freezing technique" .CHINESE CHEMICAL LETTERS 24,6(2013):491-493.

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