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Noninvasive delivery of oligonucleotide by penetratin-modified polyplexes to inhibit protein expression of intraocular tumor 期刊论文

编号：

a31fae8f-c515-456b-9fcb-5d45fb888cfd
作者：

Tai, Lingyu^[1,2];Liu, Chang^[2];Jiang, Kuan(江宽)^[2]Chen, Xishan^[2];Wei, Gang(魏刚)*^[2]Lu, Weiyue(陆伟跃)^[2]Pan, Weisan^[1];
语种：

English
期刊：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE ISSN：1549-9634 2017 年 13 卷 6 期 (2091 - 2100) ; AUG
收录：
关键词：

Intraocular drug delivery Antisense oligonucleotide Penetratin Polyplex Luciferase expression
摘要：

Our present study aimed to develop an antisense oligonucleotide (ASO) delivery system to achieve gene silencing in intraocular tumor via topical instillation. ASO specific for luciferase was chosen as model drug, polyamidoamine (PG5) was employed to condense ASO, and penetratin (Pene) was used to enhance cellular uptake. Nanoscale PG5/ASO/Pene polyplex was stabilized via noncovalent bonding. In vitro evaluations indicated that PG5/ASO/Pene exhibited improved cell-penetrating and gene silencing ability compared with naked ASO and PG5/ASO. Subcutaneous and orthotopic tumor models expressing luciferase were established in nude mice. After treated by PG5/ASO/Pene, immunohistochemical results of subcutaneous tumors showed significant inhibition of luciferase expression via peritumoral injection, and bioluminescence from orthotopic tumor was obviously weakened via topical instillation. To date, few works were successful in noninvasive treatment of intraocular diseases using antisense strategy, this penetratin-modified polyplex could be a promising vector to inhibit protein expression by effectively delivering ASOs into the eye. (c) 2017 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Tai Lingyu,Liu Chang,Jiang Kuan, et al. Noninvasive delivery of oligonucleotide by penetratin-modified polyplexes to inhibit protein expression of intraocular tumor [J].NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,2017,13(6):2091-2100.
APA：

Tai Lingyu,Liu Chang,Jiang Kuan,Chen Xishan,&Pan Weisan.(2017).Noninvasive delivery of oligonucleotide by penetratin-modified polyplexes to inhibit protein expression of intraocular tumor .NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,13(6):2091-2100.
MLA：

Tai Lingyu, et al. "Noninvasive delivery of oligonucleotide by penetratin-modified polyplexes to inhibit protein expression of intraocular tumor" .NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 13,6(2017):2091-2100.

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