The centrosomal protein gamma-tubulin complex protein 3 (Tubgcp3/GCP3) is required for the assembly of gamma-tubulin small complexes (gamma-TuSCs) and gamma-tubulin ring complexes (gamma-TuRCs), which play critical roles in mitotic spindle formation during mitosis. However, its function in vertebrate embryonic development is unknown. Here, we generated the zebrafish tubgcp3 mutants using the CRISPR/Cas9 system and found that the tubgcp3 mutants exhibited the small eye phenotype. Tubgcp3 is required for the cell cycle progression of retinal progenitor cells (RPCs), and its depletion caused cell cycle arrest in the mitotic (M) phase. The M-phase arrested RPCs exhibited aberrant monopolar spindles and abnormal distributed centrioles and gamma-tubulin. Moreover, these RPCs underwent apoptosis finally. Our study provides the in vivo model for the functional study of Tubgcp3 and sheds light on the roles of centrosomal gamma-tubulin complexes in vertebrate development.
[1]Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Zhongshan Hosp, Shanghai, Peoples R China;
[2]East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Mol Med, Sch Life Sci, Shanghai, Peoples R China
(8.0+极速模式)