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Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma 期刊论文

编号：

b0d4fe89-7f31-4b4a-b73f-862939fbb825
作者：

Wang, XiaoQing^[1,2,3];Tang, ZuXiong^[6];Yu, Dong^[7];Cui, ShuJian^[5];Jiang, YingHua(姜英华)^[1,2]Zhang, Qian^[1,2];Wang, Jie^[1,2];Yang, PengYuan(杨芃原)*^[1,2,3]Liu, Feng(刘锋)*^[1,2,4]
语种：

English
期刊：

ONCOTARGET ISSN：1949-2553 2016 年 7 卷 8 期 (8823 - 8838) ; FEB 23
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关键词：

colorectal cancer COL3A1 prognosis protein marker
摘要：

Colorectal cancer (CRC) is the third most common cancer in males and the second in females worldwide with very poor prognosis. Collagen alpha-1(III) (COL3A1) gene, encoding an extracellular matrix protein, is upregulated in human cancers. Here, we revealed that COL3A1 was increased in CRC by analysis of five Oncomine gene expression datasets (n = 496). Immunohistochemistry analysis of a tissue microarray (n = 90) demonstrated that cancer epithelial but not stromal COL3A1 was significantly upregulated comparing with the normal counterparts. High COL3A1 mRNA and/or protein expression was accompanied with high stage, T stage, Dukes stage, grade and older age, as well as smoking and recurrence status. Upregulated COL3A1 predicted poor overall (p = 0.003) and disease-free (p = 0.025) survival. Increased epithelial but not stromal COL3A1 protein predicted worse outcome (p = 0.03). Older patients (age>65) with high COL3A1 had worse survival than younger (age <= 65) with high COL3A1. Plasma COL3A1 was increased in CRC patients (n = 86) by 5.4 fold comparing with healthy individuals, enteritis and polyps patients. Plasma COL3A1 had an area under curve (AUC) of 0.92 and the best sensitivity/specificity of 98.8%/69.1%. While plasma CEA had a poorer prediction power (AUC = 0.791, sensitivity/selectivity = 70.2%/ 73.0%). Older patients (age >= 60) had higher plasma COL3A1 than younger patients. The epithelial COL3A1 protein had an AUC of 0.975 and the best sensitivity/specificity of 95.2%/91.1%. Silencing of COL3A1 suppressed CRC cell proliferation in in vitro MTT assay and in in vivo Zebra fish xenograft model by downregulation of PI3K/AKT and WNT signaling. COL3A1 was a novel diagnosis and prognosis marker of CRC.
推荐引用方式
GB/T 7714：

Wang Xiao-Qing,Tang Zu-Xiong,Yu Dong, et al. Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma [J].ONCOTARGET,2016,7(8):8823-8838.
APA：

Wang Xiao-Qing,Tang Zu-Xiong,Yu Dong,Cui Shu-Jian,&Liu Feng.(2016).Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma .ONCOTARGET,7(8):8823-8838.
MLA：

Wang Xiao-Qing, et al. "Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma" .ONCOTARGET 7,8(2016):8823-8838.

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