[1]Shanghai Med Univ 2, Ruijin Hosp, Dept Neurol, Shanghai 200025, Peoples R China
Aim To investigate the protective effect of Pituitary adenylate cyclase-activating polypeptide27 (PACAP27) on rotenone-induced cytotoxicity in PC12 cells. Methods PC12 cells differentiated by nerve growth factor as dopaminergic neurons were treated with different concentrations of rotenone and/or PACAP27, PACAP6-27; Cell viability was assessed with MTT and cell apoptosis was detected by Annexin-V staining and now cytometry. Results The cell viability decreased as the concentration of rotenone increased and this neurotoxicity was dose-dependent (P<0.01); PACAP27 (10(-12)similar to 10(-7) mol . L-1) showed significant neuroprotective effects against rotenone-indued toxicity. A decrease in the occurrence of late apoptotic features was found by double staining with Annexin-V and propidium iodide (PI) in PACAP27 group, compared with rotenone group. Flow cytometry results showed PACAP27 could improve the cell viability by decreasing the numbers of apoptotic cells, but this effects can be reversed by PACAP6-27, a kind of PACAP receptor antagonist. Conclusion These results suggest that PACAP27 may exert a protective effect against rotenone-induced neurotoxicity by PAC1 receptor in PC12 cells.
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