首页 / 院系成果 / 成果详情页

NUP153 overexpression suppresses the proliferation of colorectal cancer by negatively regulating Wnt/beta-catenin signaling pathway and predicts good prognosis 期刊论文

编号：

b4e44fd2-0c50-444d-9347-f9031a0751a6
作者：

Wu, Yibin#^[1]Fang, Guojiu^[2];Wang, Xin^[1];Wang, Huipeng^[1];Chen, Wenjie^[1];Li, Liang^[1];Ye, Tao^[1];Gong, Lifeng^[1];Ke, Chongwei(柯重伟)^[1]Cai, Yuankun(蔡元坤)*^[1]
语种：

英文
期刊：

CANCER BIOMARKERS ISSN：1574-0153 2019 年 24 卷 1 期 (61 - 70)
收录：
关键词：

NUP153 colorectal cancer suppressor gene prognosis proliferation Wnt/beta-catenin pathway
摘要：

BACKGROUND: Nucleoporin NUP153 (NUP153) is well known to be involved in the regulating of nuclear transport. Although NUP153 is associated with several cancers, its role in colorectal cancer (CRC) and the underlying mechanism are still unknown.
OBJECTIVE: The aim of this study was to access the effect of NUP153 on the prognosis of patients with CRC, and cancer cell proliferation.
METHODS: The expression levels of NUP153 in CRC tissues and matched normal colon tissues were examined by real-time quantitative PCR and immunohistochemistry. Then the association between NUP153 levels with clinical variables as well as survival time was investigated. Moreover, overexpression of NUP153 in HCT116 cells was established to study its influence on cell proliferation in vitro, and a xenograft model was performed to explore this effect in vivo.
RESULTS: We found that NUP153 was highly expressed in adjacent normal tissues than in cancer tissues, and elevated NUP153 expression was negatively associated with pathological grade (P = 0.015), T stage (P = 0.048) and distant metastasis (P = 0.006). Kaplan-Meier analysis revealed that patients with higher NUP153 expression had a longer overall survival (OS) (P = 0.01) and recurrence free disease (RFS) (P = 0.001). Logistic regression analysis further identified NUP153 as an independent prognostic safe factor for OS and recurrence. Moreover, NUP153 overexpression suppressed CRC cells proliferation and inhibited tumor growth in a xenograft model. Its mechanistic investigations showed that NUP153 overexpression inhibited beta-catenin transcriptional activity and down-regulated the mRNA expression levels of Wnt downstream proteins-Axin2, cyclinD1, c-myc and lef-1.
CONCLUSIONS: NUP153 might be a promising prognostic factor, a potential tumor suppressor and therapeutic target in human CRC through an interaction with the Wnt/beta-catenin signaling pathway.
推荐引用方式
GB/T 7714：

Wu Yibin,Fang Guojiu,Wang Xin, et al. NUP153 overexpression suppresses the proliferation of colorectal cancer by negatively regulating Wnt/beta-catenin signaling pathway and predicts good prognosis [J].CANCER BIOMARKERS,2019,24(1):61-70.
APA：

Wu Yibin,Fang Guojiu,Wang Xin,Wang Huipeng,&Cai Yuankun.(2019).NUP153 overexpression suppresses the proliferation of colorectal cancer by negatively regulating Wnt/beta-catenin signaling pathway and predicts good prognosis .CANCER BIOMARKERS,24(1):61-70.
MLA：

Wu Yibin, et al. "NUP153 overexpression suppresses the proliferation of colorectal cancer by negatively regulating Wnt/beta-catenin signaling pathway and predicts good prognosis" .CANCER BIOMARKERS 24,1(2019):61-70.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：38  下载次数：0

分享到：

浏览次数：38

下载次数：0

打印次数：0