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SPECT/CT imaging of apoptosis in aortic aneurysm with radiolabeled duramycin 期刊论文

编号：

b5093b13-1813-4f95-b680-1f150d8ecb24
作者：

Hu, Chengkai#^[1,2]Tan, Hui(谭辉)#^[3,4,5]Lin, Qingyu^[3,4,5];Abudupataer, Mieradilijiang^[1,2];Zhao, Yun(赵云)^[1,2]Li, Jun^[1,2];Gu, Jiawei(顾佳伟)^[1,2]Cheng, Dengfeng(程登峰)*^[3,4,5]Wang, Chunsheng(王春生)^[1,2]Zhu, Kai(朱铠)*^[1,2]Lai, Hao(赖颢)*^[1,2]
语种：

英文
期刊：

APOPTOSIS ISSN：1360-8185 2019 年 24 卷 9-10 期 (745 - 755) ; OCT
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关键词：

Duramycin Aortic aneurysm Apoptosis Vascular smooth muscle cells Micro-SPECT CT
摘要：

The objective of this research was to estimate whether a ([99mTc])duramycin probe can be used for apoptosis imaging in patients with aortic aneurysm (AA). Vascular smooth muscle cell (SMC) apoptosis has an important influence on AA development. Thus, non-invasive imaging of SMC apoptosis may be able to evaluate AA progress and risk stratification. SMCs were treated with hydrogen peroxide (H2O2; 200 mu M) or culture medium as a control. Apoptosis was measured using flow cytometry and ([99mTc])duramycin to detect the binding efficiency to apoptotic SMCs. C57/BL6 mice were administered angiotensin-II and beta-aminopropionitrile (BAPN) subcutaneously to establish an AA model, or saline for controls. Aortic specimens underwent pathological evaluation and their aortic diameters were measured after 6 weeks. Micro-SPECT/CT scanning of ([99mTc])duramycin and F-18-FDG PET detection were performed. SMCs treated with H2O2 showed more apoptosis compared with the control group (67.2 +/- 3.8% vs. 16.1 +/- 0.6%, P < 0.01). The experimental group showed a high rate of AA formation (70%) compared with no AA formation in the control group. The average aorta diameter was higher and ([99mTc])duramycin uptake at the AA site was higher in the experimental group compared with the control group. Compared with the normal aorta in the control group, AA in experiment group had more severe medial degeneration, elastic fiber reduction and fracture, and collagen degeneration. TUNEL staining verified the higher apoptosis rate at the AA site in experiment group compared with the control group (63.9 +/- 3.7% in ascending AA, 66.4 +/- 4.0% in thoracic AA, vs. 3.5 +/- 0.3% in normal aorta, P < 0.01). ([99mTc])Duramycin may be an effective probe to evaluate apoptosis in AA.
推荐引用方式
GB/T 7714：

Hu Chengkai,Tan Hui,Lin Qingyu, et al. SPECT/CT imaging of apoptosis in aortic aneurysm with radiolabeled duramycin [J].APOPTOSIS,2019,24(9-10):745-755.
APA：

Hu Chengkai,Tan Hui,Lin Qingyu,Abudupataer Mieradilijiang,&Lai Hao.(2019).SPECT/CT imaging of apoptosis in aortic aneurysm with radiolabeled duramycin .APOPTOSIS,24(9-10):745-755.
MLA：

Hu Chengkai, et al. "SPECT/CT imaging of apoptosis in aortic aneurysm with radiolabeled duramycin" .APOPTOSIS 24,9-10(2019):745-755.
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