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Virulence and Stress Responses of Shigella flexneri Regulated by PhoP/PhoQ 期刊论文

编号：

b94416f7-055f-4e80-b0cf-ff6469da00bb
作者：

Lin, Zhiwei^[1,2,3];Cai, Xia^[1,2,3];Chen, Mingliang^[4];Ye, Lina^[1,2,3];Wu, Yang^[1,2,3];Wang, Xiaofei(王小飞)^[1,2,3]Lv, Zhihui^[1,2,3];Shang, Yongpeng^[1,2,3];Qu, Di(瞿涤)*^[1,2,3]
语种：

English
期刊：

FRONTIERS IN MICROBIOLOGY ISSN：1664-302X 2018 年 8 卷 ; JAN 15
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关键词：

S. flexneri two-component signal transduction system PhoP/PhoQ Mg2+ virulence icsA
摘要：

The two-component signal transduction system PhoP/PhoQ is an important regulator for stress responses and virulence in most Gram-negative bacteria, but characterization of PhoP/PhoQ in Shigella has not been thoroughly investigated. In the present study, we found that deletion of phoPQ (Delta phoPQ) from Shigella flexneri 2a 301 (Sf301) resulted in a significant decline (reduced by more than 15-fold) in invasion of HeLa cells and Caco-2 cells, and less inflammation (- or +) compared to Sf301 (+++) in the guinea pig Sereny test. In low Mg2+ (10 mu M) medium or pH 5 medium, the Delta phoPQ strain exhibited a growth deficiency compared to Sf301. The.phoPQ strain was more sensitive than Sf301 to polymyxin B, an important antimicrobial agent for treating multi-resistant Gram-negative infections. By comparing the transcriptional profiles of Delta phoPQ and Sf301 using DNA microarrays, 117 differentially expressed genes (DEGs) were identified, which were involved in Mg2+ transport, lipopolysaccharide modification, acid resistance, bacterial virulence, respiratory, and energy metabolism. Based on the reported PhoP box motif [(T/G) GTTTA-5nt-(T/G) GTTTA], we screened 38 suspected PhoP target operons in S. flexneri, and 11 of them (phoPQ, mgtA, slyB, yoaE, yrbL, icsA, yhiWX, rstA, hdeAB, pagP, and shf-rfbU-virK-msbB2) were demonstrated to be PhoP-regulated genes based on electrophoretic mobility shift assays and beta-galactosidase assays. One of these PhoP-regulated genes, icsA, is a well-known virulence factor in S. flexneri. In conclusion, our data suggest that the PhoP/PhoQ system modulates S. flexneri virulence (in an icsA-dependent manner) and stress responses of Mg2+, pH and antibacterial peptides.
推荐引用方式
GB/T 7714：

Lin Zhiwei,Cai Xia,Chen Mingliang, et al. Virulence and Stress Responses of Shigella flexneri Regulated by PhoP/PhoQ [J].FRONTIERS IN MICROBIOLOGY,2018,8.
APA：

Lin Zhiwei,Cai Xia,Chen Mingliang,Ye Lina,&Qu Di.(2018).Virulence and Stress Responses of Shigella flexneri Regulated by PhoP/PhoQ .FRONTIERS IN MICROBIOLOGY,8.
MLA：

Lin Zhiwei, et al. "Virulence and Stress Responses of Shigella flexneri Regulated by PhoP/PhoQ" .FRONTIERS IN MICROBIOLOGY 8(2018).

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