Purpose: Acute renal graft rejection can only be definitively diagnosed by renal biopsy. However, biopsies carry a risk of renal transplant injury and loss. Micro-CT is widely used in preclinical studies of small animals. Here, we propose micro-CT could noninvasively monitor and evaluate renallocation and function in a mouse kidney transplant model. Methods: Orthotopic kidney transplantation was performed in a BALB/c -to- C57BL/6j or C57BL/6j-to- C57BL/6j mouse model. After optimizing imaging techniques, five mice were imaged with micro-CT and the findings were verified histologically. Results: Micro-CT can monitor and evaluate renal location and function after orthotopic kidney transplantation. There were no mice deaths while renal transplants were failure. Conclusion: We propose that graft micro-CT imaging is a new option that is noninvasive and specific, and can aid in early detection and follow-up of acute renal rejection. This method is potentially useful to improve posttransplant rejection monitoring.
[1]Fudan Univ, Huashan Hosp, Dept Urol, Shanghai 200433, Peoples R China.
[2]Natl Res Inst Child Hlth & Dev, Div Transplantat Immunol, Setagaya Ku, Tokyo 1578535, Japan.
[3]Natl Inst Infect Dis, AIDS Res Ctr, Tokyo, Japan.
(8.0+极速模式)