[1]Jilin Medical University, Tumor Targeted Therapy and Translational Medicine Laboratory,Jilin,China
[2]Jilin Medical College, Department of Pathology,Jilin,China
[3]Jilin University, Department of Hepatobiliary and Pancreas Surgery,Changchun,China
[4]Jilin Medical College, Medical Experiment Center,Jilin,China
[5]Fudan University, Pancreatic Disease Institute,Shanghai,China
[6]Jilin University, Department of Neurosurgery,Changchun,China
Objective: To isolate and culture brain tumor stem cells (BTSCs), and explore the feasibility for obtaining BTSCs by CD133 magnetic sorting and the biological characteristics of BTSCs. Methods: The fresh glioblastoma sample was dissociated, the cells were cultivated in serum-free media with growth factor, the BTSCs were purified by CD133 magnetic sorting, the positive rate of CD133+/- subgroup cells was detected by flow cytometry; neurosphere forming essay was used to analyze the self-renewal and proliferative capacity; immunofluorescent staining was performed to detect the neural stem cells (NSCs) markers CD133 and nestin of CD133+/- subgroup cells, and the multipotentiality markers β-Tubulin III and GFAP of CD133+/- subgroup cells after induction. Results: The CD133+ cells had the characteristics of stem cells, and could form the neurosphere, had the self-renewal and proliferative capacity. The expressions of CD133 and nestin were positive in CD133+ cells; β-Tubulin III and GFAP were also positive after differentiation. But CD133- cells hadn"t the characteristics mentioned above. Conclusion CD133 magnetic sorting can isolate and purify BTSCs, it can be used to get more BTSCs for experiments.
(8.0+极速模式)