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Rapid and sensitive diagnosis of porcine parvovirus by loopmediated isothermal amplification (LAMP) method 期刊论文

编号：

cc13b25a-e734-4e50-bf6c-e79101d9bbf5
作者：

Qu, Guanggang[0]^[1] Fu, Shijun[1]^[2] Shen, Ning[2]^[3] Wang, Jinliang[3]^[4] Xiao, Yueqiang[4]^[2] Guan, Yu[5]^[3] Tang, Na[6]^[3] Chen, Lei[7]^[5] Gao, Sanyang[8]^[6] Shen, Zhiqiang[9]^[2]
地址：

[1]USDA ARS Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory,Beltsville,United States
[2]Shandong Binzhou Animal Science and Veterinary Medicine Academy,,China
[3]Fudan University,Shanghai,China
[4]Shandong Binzhou Animal Science and Veterinary Medicine Institute,,China
[5]China Institute of VeterinaDrug Control,Beijing,China
[6]Shandong Lvdu Bio-Sciences and Technology Co., Ltd,,China
Scopus被引频次：

2 次
语种：

英文
期刊：

Journal of Applied Animal Research ISSN：0971-2119 2010 年 37 卷 1 期 (113 - 116)
收录：
关键词：

LAMP PCR Porcine PPV Rapid detection
摘要：

To form a detection model for the porcine parvovirus (PPV), six special primers were designed based on the PPV gene NS1. After optimization only 45 min were needed to amplify PPV DNA by incubation at 63C. The specificity of the LAMP assay was validated by the absence of any cross-reaction with six other genetically related viruses, followed by restriction digestion and was observed to be similar to PCR assay. The LAMP assay demonstrated 100-fold higher sensitivity compared to PCR, with a detection limit of 12fg DNA. Thus the LAMP can be well-applied to laboratories, as a portable device and valuable tool for differential diagnosis of PPV in the countryside.
推荐引用方式
GB/T 7714：

Qu Guanggang/55596223700[0],Fu Shijun/23484786200[1],Shen Ning/55199799700[2], et al. Rapid and sensitive diagnosis of porcine parvovirus by loopmediated isothermal amplification (LAMP) method [J].Journal of Applied Animal Research,2010,37(1):113-116.
APA：

Qu Guanggang/55596223700[0],Fu Shijun/23484786200[1],Shen Ning/55199799700[2],Wang Jinliang/55199718100[3],&Shen Zhiqiang/55462512800[9].(2010).Rapid and sensitive diagnosis of porcine parvovirus by loopmediated isothermal amplification (LAMP) method .Journal of Applied Animal Research,37(1):113-116.
MLA：

Qu Guanggang/55596223700[0], et al. "Rapid and sensitive diagnosis of porcine parvovirus by loopmediated isothermal amplification (LAMP) method" .Journal of Applied Animal Research 37,1(2010):113-116.

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