Peptide modification of nanoparticles with high efficiency is critical in determining the properties and bioapplications of nanoparticles, but the methodology remains a challenging task. Here, by using the phosphorylated linear and cyclic peptide with the arginine-glycine-aspartic acid (RGD) targeting motifs as typical examples, the peptides binding efficiency for the inorganic metal compound nanoparticles was increased significantly after the phosphorylation treatment, and the modification allowed for improving the selectivity and signal-to-noise ratio for cancer targeting and reduced the toxicity derived from nonspecific interactions of nanoparticles with cells owing to-the higher amount of phosphopeptide binding. In addition, molecular dynamics (MD) simulations of various peptides on inorganic metal compound surfaces revealed that the peptide adsorption on the surface is mainly driven by electrostatic interactions between phosphate oxygen and the polarized interfacial water layer, consistent with the experimental observation of the strong binding propensity of phosphorylated peptides. Significantly, with the RGD phosphopeptide surface modification, these nanoparticles provide a versatile tool for tuning material-cell interactions to achieve the desired level of autophagy and may prove useful for various diagnostic and therapeutic applications.
[1]Fudan Univ, State Key Lab Mol Engn Polymers, Collaborat Innovat Ctr Chem Energy Mat, Dept Chem, Shanghai 200433, Peoples R China.
[2]Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China.
[3]Fudan Univ, Sch Pharm, Shanghai 201203, Peoples R China.
[4]Macquarie Univ, ARC Ctr Excellence Nanoscale BioPhoton CNBP, Adv Cytometry Labs, Sydney, NSW 2109, Australia.
[5]King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia.
[6]Cent Met Res & Dev Inst, Cairo 11421, Egypt.
(8.0+极速模式)