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Rescue of Photoreceptors by BDNF Gene Transfer Using In Vivo Electroporation in the RCS Rat of Retinitis Pigmentosa 期刊论文

编号：

d6431072-fa5a-4e37-b70f-4eb730428038
作者：

Zhang, Meng^[1];Mo, Xiaofen^[1];Fang, Yuan^[1];Guo, Wenyi(郭文毅)^[1]Wu, Jihong^[1];Zhang, Shenghai^[1];Huang, Qian^[2];
语种：

English
期刊：

CURRENT EYE RESEARCH ISSN：0271-3683 2009 年 34 卷 9 期 (791 - 799)
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关键词：

electroporation gene therapy retinal ganglion cell retinal pigment epithelium retinitis pigmentosa
摘要：

Purpose: To investigate the feasibility of introducing brain-derived neurotrophic factor (BDNF) gene into retinal pigment epithelial cells in vivo by electroporation and whether this method can rescue photoreceptors of retinitis pigmentosa in Royal College Surgeons (RCS) rats. Methods: The BDNF-GFP fusion eukaryotic-expressing plasmid was constructed and subretinally or intravitreously injected into the eyes of RCS rats followed by in vivo electroporation. The expression of BDNF mRNA and protein was detected by RT-PCR and Western immunoblot analysis. The number of surviving photoreceptors was counted, and the TdT-dUTP terminal nick-end labeling (TUNEL) method was used to detect the apoptotic retinal cells at different timepoints after introduction of BDNF plasmid. Results: Treated eyes showed a significantly higher rescue ratio and a lower number of TUNEL-positive photoreceptors than did the control eyes at various timepoints. Conclusion: These findings provide evidence that electroporation is an effective method for gene transfer into retinal pigment epithelial cells, and the rescue of photoreceptors can be achieved by BDNF gene transfection with electroporation.
推荐引用方式
GB/T 7714：

Zhang Meng,Mo Xiaofen,Fang Yuan, et al. Rescue of Photoreceptors by BDNF Gene Transfer Using In Vivo Electroporation in the RCS Rat of Retinitis Pigmentosa [J].CURRENT EYE RESEARCH,2009,34(9):791-799.
APA：

Zhang Meng,Mo Xiaofen,Fang Yuan,Guo Wenyi,&Huang Qian.(2009).Rescue of Photoreceptors by BDNF Gene Transfer Using In Vivo Electroporation in the RCS Rat of Retinitis Pigmentosa .CURRENT EYE RESEARCH,34(9):791-799.
MLA：

Zhang Meng, et al. "Rescue of Photoreceptors by BDNF Gene Transfer Using In Vivo Electroporation in the RCS Rat of Retinitis Pigmentosa" .CURRENT EYE RESEARCH 34,9(2009):791-799.

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