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Human Bex2 interacts with LMO2 and regulates the transcriptional activity of a novel DNA-binding complex 期刊论文

编号：

d7ffe7a3-ca1e-43a6-8229-d47a248122ed
作者：

Han, CY; Liu, H; Liu, J; Yin, K; Xie, Y; Shen, X; Wang, Y; Yuan, JG; Qiang, BQ; Liu, YJ; Peng, XZ
语种：

English
期刊：

NUCLEIC ACIDS RESEARCH ISSN：0305-1048 2005 年 33 卷 20 期 (6555 - 6565)
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摘要：

Human Bex2 (brain expressed X-linked, hBex2) is highly expressed in the embryonic brain, but its function remains unknown. We have identified that LMO2, a LIM-domain containing transcriptional factor, specifically interacts with hBex2 but not with mouse Bex1 and Bex2. The interaction was confirmed both by pull-down with GST-hBex2 and by coimmunoprecipitation assays in vivo. Using electrophoretic mobility shift assay, we have demonstrated the physical interaction of hBex2 and LMO2 as part of a DNA-binding protein complex. We have also shown that hBex2 can enhance the transcriptional activity of LMO2 in vivo. Furthermore, using mammalian two-hybrid analysis, we have identified a neuronal bHLH protein, NSCL2, as a novel binding partner for LMO2. We then showed that LMO2 could up-regulate NSCL2-dependent transcriptional activity, and hBex2 augmented this effect. Thus, hBex2 may act as a specific regulator during embryonic development by modulating the transcriptional activity of a novel E-box sequence-binding complex that contains hBex2, LMO2, NSCL2 and LDB1.
推荐引用方式
GB/T 7714：

Han CY,Liu H,Liu J, et al. Human Bex2 interacts with LMO2 and regulates the transcriptional activity of a novel DNA-binding complex [J].NUCLEIC ACIDS RESEARCH,2005,33(20):6555-6565.
APA：

Han CY,Liu H,Liu J,Yin K,&Peng XZ.(2005).Human Bex2 interacts with LMO2 and regulates the transcriptional activity of a novel DNA-binding complex .NUCLEIC ACIDS RESEARCH,33(20):6555-6565.
MLA：

Han CY, et al. "Human Bex2 interacts with LMO2 and regulates the transcriptional activity of a novel DNA-binding complex" .NUCLEIC ACIDS RESEARCH 33,20(2005):6555-6565.

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