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MicroRNA-17 inhibition overcomes chemoresistance and suppresses epithelial-mesenchymal transition through a DEDD-dependent mechanism in gastric cancer 期刊论文

编号：

d80d4e7e-aa1b-4907-9a71-1be9ba0a4fee
作者：

Wu, DongMei^[1,2];Hong, XiaoWu(洪晓武)^[3]Wang, LingLing^[1];Cui, XiaFeng^[1];Lu, Jun^[1,2];Chen, GuiQuan^[4];Zheng, YuanLin^[1,2];
语种：

English
期刊：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY ISSN：1357-2725 2018 年 102 卷 (59 - 70) ; SEP
收录：
关键词：

MicroRNA-17 Death effector domain-containing DNA-binding protein Epithelial-mesenchymal transition Chemosensitivity Gastric cancer
摘要：

MicroRNAs (miRNAs), a novel class of important gene-regulatory molecules, correlates with tumor growth, invasion, metastasis, and chemo resistance in gastric cancer (GC). Microarray analysis revealed that aberrant expressed microRNA-17 (miR-17) and DEDD were identified in GC. DEDD has been found to act as an endogenous suppressor of tumor growth and metastasis through epithelial-mesenchymal transition (EMT) process. However, the role of miRNA-17 (miR-17) has not been clearly evaluated in GC, thereby a series of in vitro experiments were performed in this study. The levels of miR-17 and DEDD in GC tissues from patients diagnosed with GC and in five GC cell lines (SGC-7901, MKN-45, HGC-27, BGC823, and AGS) were detected. It was found that miR-17 up-regulated and DEDD down-regulated in GC, and SGC-7901 and AGS cells were adopted for the in vitro cell experiments, in which the expression of miR-17 or DEDD was regulated by transfection. DEDD was validated to be a target gene of miR-17. Inhibition of miR-17 impaired EMT in GC cells. In addition, transwell assay and scratch test results revealed that inhibition of miR-17 hindered GC cell invasion and migration. Moreover, inhibition of miR-17 reduced resistance to cisplatin- or 5-Fu in GC cells and induced cisplatin- or 5-Futreated GC cell apoptosis, which evaluated by using CCK-8 and flow cytometry assays. From the short review above, the key findings emerge that inhibition of miR-17 may have tumor suppressive effects on GC and enhance its chemosensitivity by promoting DEDD, highlighting a novel target for GC therapy.
推荐引用方式
GB/T 7714：

Wu Dong-Mei,Hong Xiao-Wu,Wang Ling-Ling, et al. MicroRNA-17 inhibition overcomes chemoresistance and suppresses epithelial-mesenchymal transition through a DEDD-dependent mechanism in gastric cancer [J].INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2018,102:59-70.
APA：

Wu Dong-Mei,Hong Xiao-Wu,Wang Ling-Ling,Cui Xia-Feng,&Zheng Yuan-Lin.(2018).MicroRNA-17 inhibition overcomes chemoresistance and suppresses epithelial-mesenchymal transition through a DEDD-dependent mechanism in gastric cancer .INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,102:59-70.
MLA：

Wu Dong-Mei, et al. "MicroRNA-17 inhibition overcomes chemoresistance and suppresses epithelial-mesenchymal transition through a DEDD-dependent mechanism in gastric cancer" .INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 102(2018):59-70.

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