首页 / 院系成果 / 成果详情页

In vivo serial MR imaging of magnetically labeled endothelial progenitor cells homing to the endothelium injured artery in mice 期刊论文

编号：

d89e515c-d909-432d-81d3-7ca3ad149285
作者：

Chen, Jun[0]^[1] Jia, Zhenyu[1]^[2] Teng, Gaojun[2]^[3] Ma, Zhanlong[3]^[4] Wang, Yuanyuan[4]^[5]
地址：

[1]Capital Medical University China, Institute for Biomedical Sciences of Pain,Beijing,China
[2]Hospital of Nanjing Medical University, Department of Radiology,Nanjing,China
[3]Southeast University, School of Medicine,Nanjing,China
[4]s Hospital, Department of Radiology,Yangzhou,China
[5]Fudan University, Department of Electronics Engineering,Shanghai,China
语种：

英文
期刊：

PLoS ONE ISSN：1932-6203 2011 年 6 卷 6 期
收录：
摘要：

Background: Emerging evidence of histopathological analyses suggests that endothelial progenitor cells (EPCs) play an important role in vascular diseases. Neointimal hyperplasia can be reduced by intravenous transfusion of EPCs after vascular injury in mice. Therefore, it would be advantageous to develop an in vivo technique that can explore the temporal and spatial migration of EPCs homing to the damaged endothelium noninvasively. Methodology/Principal Findings: The left carotid common artery (LCCA) was injured by removal of endothelium with a flexible wire in Kunming mice. EPCs were collected by in vitro culture of spleen-derived mouse mononuclear cells (MNCs). EPCs labeling was carried out in vitro using Fe₂O₃-poly-L-lysine (Fe₂O₃-PLL). In vivo serial MR imaging was performed to follow-up the injured artery at different time points after intravenous transfusion of EPCs. Vessel wall areas of injured artery were computed on T₂WI. Larger MR signal voids of vessel wall on T₂WI was revealed in all 6 mice of the labeled EPC transfusion group 15 days after LCCA injury, and it was found only in 1 mouse in the unlabeled EPC transfusion group (p = 0.015). Quantitative analyses of vessel wall areas on T₂WI showed that the vessel wall areas of labeled EPC transfusion group were less than those of unlabeled EPC transfusion group and control group fifteen days after artery injury (p<0.05). Histopathological analyses confirmed accumulation and distribution of transfused EPCs at the injury site of LCCA. Conclusions/Significance: These data indicate that MR imaging might be used as an in vivo method for the tracking of EPCs homing to the endothelium injured artery. ? 2011 Chen et al.
推荐引用方式
GB/T 7714：

Chen Jun/7501883403[0],Jia Zhenyu/55382440200[1],Teng Gaojun/7004411906[2], et al. In vivo serial MR imaging of magnetically labeled endothelial progenitor cells homing to the endothelium injured artery in mice [J].PLoS ONE,2011,6(6).
APA：

Chen Jun/7501883403[0],Jia Zhenyu/55382440200[1],Teng Gaojun/7004411906[2],Ma Zhanlong/15849048900[3],&Wang Yuanyuan/55734078400[4].(2011).In vivo serial MR imaging of magnetically labeled endothelial progenitor cells homing to the endothelium injured artery in mice .PLoS ONE,6(6).
MLA：

Chen Jun/7501883403[0], et al. "In vivo serial MR imaging of magnetically labeled endothelial progenitor cells homing to the endothelium injured artery in mice" .PLoS ONE 6,6(2011).

浏览次数：1  下载次数：0

分享到：

浏览次数：1

下载次数：0

打印次数：0