Aim: To develop a novel non-viral vector with high transfection efficiency and low cytotoxicity. Methods: Poly (ethylene glycol)-distearoylphosphatidylethanolamine (PEG-DSPE) was incorporated into polymer-lipid hybrid nanoparticles (PLN) to construct a PEG-DSPE modified long circulating PLN (L-PLN). The L-PLN was prepared by the emulsifying-solvent evaporation method, L-PLN and L-PLN/DNA complexes were characterized. Both HEK293 and MDA-MB-231 cells transfected by L-PLN/DNA complexes were observed under a fluorescence microscope. The transfection efficiency of the complexes to HEK293 cells was further evaluated by flow cytometry. Results: The GFP fluorescence intensity in HEK293 cells transfected by the L-PLN/DNA complexes (N/P=10) was about 37.2%, which was higher than those transfected by PLN alone or commercial Lipofectamine (TM) 2000. The L-PLN exhibited minimal toxicity at a low N/P ratio compared with other vectors. Conclusion: L-PLN as a novel gene delivery system, has higher transfection efficiency and acceptable cytotoxicity compared to the corresponding PLN, which is beneficial for the development of non-viral gene transfer vectors and may offer an alternative strategy for the future gene therapy.
[1]Harbin Med Coll, Dept Otolaryngol, Hosp 1, Harbin 150001, Peoples R China.
[2]Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China.
[3]Fudan Univ, Affiliated Eye & ENT Hosp, Dept Otolaryngol, Shanghai 200031, Peoples R China.
(8.0+极速模式)