首页 / 院系成果 / 成果详情页

GSK-3 beta signaling pathway in an in vivo model of cerebral ischemia 期刊论文

编号：

daefc10c-1858-4e84-88b7-8fca92031e8d
作者：

Tao, Jinhao^[1];Cui, Yuehua^[3,4];Duan, Yu(段宇)^[3]Zhang, Nan(张南)^[2]Wang, Congmin^[5];Zhang, Fayong(张法永)*^[2]
语种：

English
期刊：

ONCOTARGET ISSN：1949-2553 2017 年 8 卷 63 期 (106283 - 106295) ; DEC 5
收录：
关键词：

cerebral ischemia/reperfusion puerarin hippocampus Akt GSK-3 beta
摘要：

Ischemic stroke causes irreversible damage to the brain. The hippocampus is a vulnerable region and plays an important role in cognition and locomotor activity. Puerarin is a phytoestrogen that has beneficial effects in treating neurological disorders. How puerarin protects against hippocampal injury and its molecular mechanisms remain to be elucidated. Transient global brain ischemia was induced by 4-vessel occlusion in adult male Sprague-Dawley rats. The rats were pretreated with puerarin alone or together with LY294002 (an PI3K inhibitor) before ischemia/ reperfusion (I/R). The open-and closed-field tasks and Morris water maze (MWM) test were used to assess the effects of puerarin on anxiety-like behavioral and cognitive impairment following I/R. Hematoxylin-eosin staining(HE) was used to examine the survival of hippocampal CA1 pyramidal neurons, and immunoblotting was performed to examine the expression of the related proteins. By using the rat model for transient I/R, we demonstrated that puerarin pretreatment significantly increased the travelling distance and number of crossings in the open-and closedfield tests, reduced latency and increased the proportion of distance and time in zone IV in the MWM. The number of live cells in the hippocampus is sharply increased by puerarin pretreatment. We further observed that the levels of phosphorylated Akt1, GSK-3 beta and MCL-1were elevated and those of cleaved-caspase-3 were reduced in the puerarin-treatment group. Notably, the PI3K inhibitor LY294002 counteracted all of the effects of puerarin. Our findings suggest that puerarin protects the hippocampus from I/R damage by activating the PI3K/Akt1/GSK-3 beta/MCL-1 signaling pathway.
推荐引用方式
GB/T 7714：

Tao Jinhao,Cui Yuehua,Duan Yu, et al. Puerarin attenuates locomotor and cognitive deficits as well as hippocampal neuronal injury through the PI3K/Akt1/GSK-3 beta signaling pathway in an in vivo model of cerebral ischemia [J].ONCOTARGET,2017,8(63):106283-106295.
APA：

Tao Jinhao,Cui Yuehua,Duan Yu,Zhang Nan,&Zhang Fayong.(2017).Puerarin attenuates locomotor and cognitive deficits as well as hippocampal neuronal injury through the PI3K/Akt1/GSK-3 beta signaling pathway in an in vivo model of cerebral ischemia .ONCOTARGET,8(63):106283-106295.
MLA：

Tao Jinhao, et al. "Puerarin attenuates locomotor and cognitive deficits as well as hippocampal neuronal injury through the PI3K/Akt1/GSK-3 beta signaling pathway in an in vivo model of cerebral ischemia" .ONCOTARGET 8,63(2017):106283-106295.

浏览次数：3  下载次数：0

分享到：

浏览次数：3

下载次数：0

打印次数：0