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Helix B surface peptide attenuates diabetic cardiomyopathy via AMPK-dependent autophagy 期刊论文

编号：

e40b091a-ac08-4bca-8042-fb940c4f630d
作者：

Lin, Chen^[1];Zhang, Mingming^[1];Zhang, Yingmei^[2]Yang, Kejian^[1];Hu, Jiangiang^[1];Si, Rui^[1];Zhang, Guoyong^[1];Gao, Beilei^[1];Li, Xiang^[1];Xu, Chennian^[1];Li, Congye^[1];Hao, Qimeng^[3];Guo, Wenyi^[1];
语种：

English
期刊：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ISSN：0006-291X 2017 年 482 卷 4 期 (665 - 671) ; JAN 22
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关键词：

Diabetic cardiomyopathy Helix B surface peptide Apoptosis Autophagy
摘要：

Background: Erythropoietin (EPO) has been reported to exert protective effects on a host of damaged tissues. However, the erythropoietic effect of this hormone can result in high risks of thrombosis, stroke, and hypertension, remarkably limiting the clinical use of EPO. Helix B surface peptide (HBSP) is a small peptide derived from the helix-B domain of EPO. Surprisingly, HBSP retains the tissue protective properties of EPO without altering the hematocrit. Thus, we evaluated the possible role of HBSP on diabetic cardiomyopathy. Methods: Diabetes was induced in mice by intraperitoneal injections of streptozocin (STZ). Mice were randomly treated with normal saline or HBSP. Cardiac function, fibrosis, apoptosis, and myocardial mitochondrial morphology were examined. For in vitro experiments, H9C2 myoblast cells were randomly grouped as normal glucose (NG, 5 mM), NG+HBSP (100 nM), high glucose (HG, 33 mM), HG+HBSP (100 nM), HG+HBSP+3-methyladenine (3-MA, 10 mM), HG+rapamycin (Rapa, 100 nM), and HG+HBSP+Compound C (CC, 10 mM). Autophagosomes, LC3 dots, apoptosis and mitochondria membrane potential (MMP) of H9C2 cells were examined.The expressions of LC3, p62, p-AMPK (Thr172) and p-mTOR (Ser2448) were examined by Western blot. Results: HBSP markedly improved cardiac function, attenuated cardiac interstitial fibrosis, inhibited myocardial apoptosis, and ameliorated mitochondrial ultrastructure in mice with diabetic cardiomyopathy. HG reduced autophagy in H9C2 cells. HBSP enhanced autophagy in HG-treated H9C2 cells. HBSP reduced the apoptosis index of HG-treated H9C2 cells. HBSP increased the MMP of HG-treated H9C2 cells. HBSP increased the levels of p-AMPK (Thr172), and reduced p-mTOR (Ser2448) in HG-treated H9C2 cells, and the increase of p-AMPK(Thr172) was accompanied by the stimulation of autophagy. Autophagy inhibitor 3-MA and AMPK inhibitor CC mitigated HBSP-induced beneficial effect, whereas autophagy inducer Rapa alleviated the HG-induced cell apoptosis. Conclusions: HBSP attenuates diabetic cardiomyopathy via autophagy mediated by AMPK-dependent pathway. HBSP may be a potential therapeutic intervention for diabetic cardiomyopathy. (C) 2016 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Lin Chen,Zhang Mingming,Zhang Yingmei, et al. Helix B surface peptide attenuates diabetic cardiomyopathy via AMPK-dependent autophagy [J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2017,482(4):665-671.
APA：

Lin Chen,Zhang Mingming,Zhang Yingmei,Yang Kejian,&Guo Wenyi.(2017).Helix B surface peptide attenuates diabetic cardiomyopathy via AMPK-dependent autophagy .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,482(4):665-671.
MLA：

Lin Chen, et al. "Helix B surface peptide attenuates diabetic cardiomyopathy via AMPK-dependent autophagy" .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 482,4(2017):665-671.

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