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Mannose receptor modulates macrophage polarization and allergic inflammation through miR-511-3p 期刊论文

编号：

f35fc018-1ff0-440c-be13-ecdfa28667f4
作者：

Zhou, Yufeng(周玉峰)^[1,8,9,10]Do, Danh C.^[1];Ishmael, Faoud T.^[4];Squadrito, Mario Leonardo^[5];Tang, Ho Man^[2];Tang, Ho Lam^[6];Hsu, ManHsun^[4];Qiu, Lipeng^[1];Li, Changjun^[3];Zhang, Yongqing^[7];Becker, Kevin G.^[7];Wan, Mei^[3];Huang, ShauKu^[1,11,12,13];Gao, Peisong^[1];
语种：

English
期刊：

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY ISSN：0091-6749 2018 年 141 卷 1 期 (350 - +) ; JAN
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关键词：

Mannose receptor miR-511-3p macrophage asthma
摘要：

BACKGROUND: Mannose receptor (MRC1/CD206) has been suggested to mediate allergic sensitization and asthma to multiple glycoallergens, including cockroach allergens. OBJECTIVE: We sought to determine the existence of a protective mechanism through which MRC1 limits allergic inflammation through its intronic miR-511-3p. METHODS: We examined MRC1-mediated cockroach allergen uptake by lung macrophages and lung inflammation using C57BL/6 wild-type (WT) and Mrc1(-/-) mice. The role of miR-511-3p in macrophage polarization and cockroach allergen-induced lung inflammation in mice transfected with adeno-associated virus (AAV)-miR-511-3p (AAV-cytomegalovirus-miR-511-3p-enhanced green fluorescent protein) was analyzed. Gene profiling of macrophages with or without miR-511-3p overexpression was also performed. RESULTS: Mrc1(-/-) lung macrophages showed a significant reduction in cockroach allergen uptake compared with WT mice, and Mrc1(-/-) mice had an exacerbated lung inflammation with increased levels of cockroach allergen-specific IgE and T(H)2/T(H)17 cytokines in a cockroach allergen-induced mouse model compared with WT mice. Macrophages from Mrc1(-/-) mice showed significantly reduced levels of miR-511-3 and an M1 phenotype, whereas overexpression of miR-511-3p rendered macrophages to exhibit a M2 phenotype. Furthermore, mice transfected with AAV-miR-511-3p showed a significant reduction in cockroach allergen-induced inflammation. Profiling of macrophages with or without miR-511-3p overexpression identified 729 differentially expressed genes, wherein expression of prostaglandin D2 synthase (Ptgds) and its product PGD2 were significantly downregulated by miR-511-3p. Ptgds showed a robust binding to miR-511-3p, which might contribute to the protective effect of miR-511-3p. Plasma levels of miR-511-3p were significantly lower in human asthmatic patients compared with nonasthmatic subjects. CONCLUSION: These studies support a critical but previously unrecognized role of MRC1 and miR-511-3p in protection against allergen-induced lung inflammation.
推荐引用方式
GB/T 7714：

Zhou Yufeng,Do Danh C.,Ishmael Faoud T., et al. Mannose receptor modulates macrophage polarization and allergic inflammation through miR-511-3p [J].JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY,2018,141(1):350-+.
APA：

Zhou Yufeng,Do Danh C.,Ishmael Faoud T.,Squadrito Mario Leonardo,&Gao Peisong.(2018).Mannose receptor modulates macrophage polarization and allergic inflammation through miR-511-3p .JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY,141(1):350-+.
MLA：

Zhou Yufeng, et al. "Mannose receptor modulates macrophage polarization and allergic inflammation through miR-511-3p" .JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 141,1(2018):350-+.

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